Blueprints for green biotech: development and application of standards for plant synthetic biology.

Blueprints for green biotech: development and application of standards for plant synthetic biology.

Synthetic biology goals to use engineering ideas to the design and modification of organic programs and to the development of organic components and gadgets. The capacity to programme cells by offering new directions written in DNA is a foundational know-how of the sphere. Large-scale de novo DNA synthesis has accelerated synthetic biology by providing custom-made molecules at ever lowering prices. However, for massive fragments and for experiments during which libraries of DNA sequences are assembled in several mixtures, meeting within the laboratory continues to be fascinating.

Biological meeting standards permit DNA components, even these from a number of laboratories and experiments, to be assembled collectively utilizing the identical reagents and protocols. The adoption of such standards for plant synthetic biology has been cohesive for the plant science neighborhood, facilitating the application of genome enhancing applied sciences to plant programs and streamlining progress in large-scale, multi-laboratory bioengineering initiatives. This perspective will display the shift that biotech/pharma is already making and will suggest that the trade has a chance to compel change by adopting some points of the tech trade on the subject of fashions for know-how/product development and management attributes.

Subjects had been divided into 4 teams: Group 1 included 140 diabetic and non-diabetic topics with regular urea and haemoglobin ranges; Group 2 included 84 diabetic and non-diabetic topics with excessive urea ranges; Group three included 44 diabetic and non-diabetic topics with iron deficiency anaemia; and Group four included 52 diabetic and non-diabetic topics with excessive haemoglobin ranges. EP Evaluator Release eight program was used to guage the resultant information. The biotech and pharma (biotech/pharma) trade is in dire want of discovering methods to extend efficacy, effectivity, and affordability of its merchandise. The info and know-how trade (“tech”) trade, which is an trade equally based on innovations and innovation, might present some meals for thought.

 Blueprints for green biotech: development and application of standards for plant synthetic biology.

RABiT-II: Implementation of a High-Throughput Micronucleus Biodosimetry Assay on Commercial Biotech Robotic Systems.

We display the use of high-throughput biodosimetry platforms primarily based on industrial high-throughput/high-content screening robotic programs. The cytokinesis-block micronucleus (CBMN) assay, utilizing solely 20 μl complete blood from a fingerstick, was applied on a PerkinElmer cell::explorer and General Electric IN Cell Analyzer 2000. On common 500 binucleated cells per pattern had been detected by our FluorQuantMN software program. A calibration curve was generated within the radiation dose vary as much as 5.0 Gy utilizing the information from eight donors and 48,083 binucleated cells in complete.
The research described right here demonstrates that high-throughput radiation biodosimetry is sensible utilizing present industrial high-throughput/high-content screening robotic programs, which may be readily programmed to carry out and analyze robotics-optimized cytogenetic assays. Application to different industrial high-throughput/high-content screening programs past those used on this research is clearly sensible. This method will permit a lot wider entry to high-throughput biodosimetric screening for large-scale radiological incidents than is at present accessible.
Keep up with at the moment’s single-use know-how customary but in addition stay one step forward” – that would have been the motto of the BioTech 2016 convention at ZHAW Waedenswil on September 5 and 6, which introduced collectively producers, suppliers, customers, regulatory specialists and representatives of organizations on this discipline to implement single-use gadgets everywhere in the world. It was an effectiveness evaluation of the pattern to exchange reusable gear in manufacturing amenities with single-use elements.
Most of the genetically modified (GM) crops at present commercialized embody a handful of crop species (soybean, corn, cotton and canola) with agronomic characters (traits) directed in opposition to some biotic stresses (pest resistance, herbicide tolerance or each) and created by multinational corporations. The identical crops with agronomic traits already available on the market at the moment will proceed to be commercialized, however there can be additionally a wider vary of species with mixed traits.
The timeframe anticipated for market launch of the following biotech crops is not going to solely depend upon science progress in analysis and development (R&D) in laboratories and fields, but in addition totally on how demanding regulatory necessities are in international locations the place advertising and marketing approvals are pending. Regulatory constraints, together with environmental and well being impression assessments, have elevated considerably previously a long time, delaying approvals and rising their prices. This has generally discouraged public analysis entities and small and medium measurement plant breeding corporations from utilizing biotechnology and given desire to different applied sciences, not as stringently regulated.
Nevertheless, R&D applications are flourishing in growing international locations, boosted by the need to satisfy the worldwide challenges which are meals safety of a booming world inhabitants whereas mitigating local weather change impacts. Biotechnology is an instrument on the service of these imperatives and all kinds of crops are at present examined for their excessive yield regardless of biotic and abiotic stresses. Many crops with greater water or nitrogen use effectivity, tolerant to chilly, salinity or water submergence are being developed. Food safety is just not solely a query of amount but in addition of high quality of agricultural and meals merchandise, to be accessible and accessible for those who want it probably the most.
Many biotech crops (particularly staple meals) are subsequently being developed with dietary traits, akin to biofortification in nutritional vitamins and metals. The principal worldwide seed corporations proceed to be the most important buyers in plant biotechnology R&D, and usually collaborate within the growing world with public establishments, personal entities and philanthropic organizations. These partnerships are significantly current in Africa. In developed international locations, plant biotechnology can also be used for non-food functions, such because the pharmaceutical, biofuel, starch, paper and textile industries.

Protease Activity Assay Kit

AR4011-unit unit
EUR 340

Trypsin Activity Assay Kit

3043 1 kit
EUR 483.55
Description: Trypsin Activity Assay Kit

MAT Activity Assay Kit

abx098901-96tests 96 tests
EUR 1193

MAT Activity Assay

IK00401 96 tests
EUR 844
Description: Methionine Adenosyltransferase (MAT) activity assay kit

GST Colorimetric Activity Assay Kit

55R-1353 100 assays
EUR 706
Description: Assay Kit for detection of GST activity in the research laboratory

Beta Secretase Activity Assay Kit

55R-1383 100 assays
EUR 835
Description: Assay Kit for detection of Beta Secretase Activity in the research laboratory

Alkaline Phosphatase Activity Assay Kit

55R-1402 500 assays
EUR 740
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

Acid Phosphatase Activity Assay Kit

55R-1405 500 assays
EUR 438
Description: Assay Kit for detection of Acid Phosphatase in the research laboratory

Alkaline Phosphatase Activity Assay Kit

55R-1406 500 assays
EUR 438
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

Acid Phosphatase Activity Assay Kit

55R-1866 500 assays
EUR 484
Description: Assay Kit for detection of Acid Phosphatase in the research laboratory

Neprilysin Activity Assay Kit (Fluorometric)

K487-100
EUR 610

Chitotriosidase Activity Assay Kit (Fluorometric)

K512-100
EUR 620

?-Glucuronidase Activity Assay Kit (Fluorometric)

K514-100
EUR 620

Methyltransferase Activity Assay Kit (Fluorometric)

K521-100
EUR 566

Transglutaminase Activity Assay Kit (Colorimetric)

K571-100
EUR 718

Sphingomyelinase Activity Fluorometric Assay Kit

K574-100
EUR 457

Fumarase Activity Colorimetric Assay Kit

K596-100
EUR 653

Sphingomyelinase Activity Colorimetric Assay Kit

K599-100
EUR 446

Invertase Activity Colorimetric Assay Kit

K674-100
EUR 490

Sulfatase Activity Assay Kit (Colorimetric)

K675-100
EUR 653

Arginase Activity Colorimetric Assay Kit

K755-100
EUR 539

Acetylcholinesterase Activity Colorimetric Assay Kit

K764-100
EUR 550

Trypsin Activity Colorimetric Assay Kit

K771-100
EUR 452

DPP4 Activity Fluorometric Assay Kit

K779-100
EUR 544

Protease Activity Fluorometric Assay Kit

K781-100
EUR 387

Collagenase Activity Colorimetric Assay Kit

K792-100
EUR 408

Renin Activity Fluorometric Assay Kit

K800-100
EUR 523

Plasmin Activity Assay Kit (Colorimetric)

K945-100
EUR 446

Cholinesterase Activity Assay Kit (Colorimetric)

K975-100
EUR 642

Lipoxygenase Activity Assay Kit (Fluorometric)

K978-100
EUR 653

?-Xylosidase Activity Assay Kit (Fluorometric)

K981-100
EUR 588

Methyltransferase Activity Assay Kit (Colorimetric)

K986-100
EUR 648

Tyrosinase Activity Assay Kit (Colorimetric)

K742-100
EUR 582

?-Glucosidase Activity Colorimetric Assay Kit

K690-100
EUR 550

Aconitase Activity Colorimetric Assay Kit

K716-100
EUR 501

Urokinase Activity Fluorometric Assay Kit

K728-100
EUR 544

Lipase Activity Colorimetric Assay Kit

K722-100
EUR 512

Neuraminidase Activity Fluorometric Assay Kit

K732-100
EUR 523

Uricase Activity Assay Kit (Fluorometric)

K734-100
EUR 512

Amylase Activity Colorimetric Assay Kit

K711-100
EUR 408

Deubiquitinase Activity Assay Kit (Fluorometric)

K485-100
EUR 533

HDAC6 Activity Assay Kit (Fluorometric)

K466-100
EUR 631

Aldolase Activity Colorimetric Assay Kit

K665-100
EUR 664

Ceruloplasmin Activity Colorimetric Assay Kit

K669-100
EUR 550

GST Colorimetric Activity Assay Kit

K263-100
EUR 490

GST Fluorometric Activity Assay Kit

K260-100
EUR 468

Lysosomal Intracellular Activity Assay Kit

K448-50
EUR 544

Akt Activity Assay Kit, KinaseSTAR

K435-40
EUR 680

JNK Activity Assay Kit, KinaseSTAR

K431-40
EUR 664

Chymotrypsin Activity Assay Kit (Fluorometric)

K352-100
EUR 490

HAT Activity Colorimetric Assay Kit

K332-100
EUR 539

HAT Activity Fluorometric Assay Kit

K334-100
EUR 539

HDAC3 Activity Fluorometric Assay Kit

K343-100
EUR 479

Sirtuin Activity Assay Kit (Fluorometric)

K324-100
EUR 572

HDAC Activity Fluorometric Assay Kit

K330-100
EUR 490

HDAC Activity Colorimetric Assay Kit

K331-100
EUR 490

Plasmin Activity Assay Kit (Fluorometric)

K381-100
EUR 457

Thrombin Activity Fluorometric Assay Kit

K373-100
EUR 697

Urease Activity Assay Kit (Colorimetric)

K378-100
EUR 501

Calpain Activity Fluorometric Assay Kit

K240-100
EUR 588

Proteasome Activity Fluorometric Assay Kit

K245-100
EUR 479

Lysozyme Activity Assay Kit (Fluorometric)

K236-100
EUR 490

HDAC8 Activity Fluorometric Assay Kit

K348-100
EUR 490

ATPase Activity Assay Kit (Colorimetric)

K417-100
EUR 430

Caspase-1 Activity Assay Kit

AR4003-100Assays 100 Assays
EUR 514

Caspase-1 Activity Assay Kit

AR4003-25Assays 25 Assays
EUR 236

Caspase-1 Activity Assay Kit

AR4003-50Assays 50 Assays
EUR 340

Caspase-2 Activity Assay Kit

AR4004-100Assays 100 Assays
EUR 514

Caspase-2 Activity Assay Kit

AR4004-25Assays 25 Assays
EUR 236

Caspase-2 Activity Assay Kit

AR4004-50Assays 50 Assays
EUR 340

DAG Kinase Activity Assay Kit

MET-5036 50 assays
EUR 450

Hexokinase (HK) Activity Assay Kit

MET-5087 100 assays
EUR 508

Beta Hexosaminidase Activity Assay Kit

MET-5095 100 assays
EUR 508

OxiSelect ORAC Activity Assay Kit

STA-345 192 assays
EUR 577
Description: The ORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect ORAC Activity Assay measures this capacity in less than 2 hours from a wide variety of sample types.

OxiSelect ORAC Activity Assay Kit

STA-345-5 5 x 192 assays
EUR 2277
Description: The ORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect ORAC Activity Assay measures this capacity in less than 2 hours from a wide variety of sample types.

OxiSelect HORAC Activity Assay Kit

STA-346 192 assays
EUR 577
Description: The HORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect HORAC Activity Assay measures the degradation of free hydroxyl radicals in less than 2 hours from a wide variety of sample types.

OxiSelect HORAC Activity Assay Kit

STA-346-5 5 x 192 assays
EUR 2277
Description: The HORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect HORAC Activity Assay measures the degradation of free hydroxyl radicals in less than 2 hours from a wide variety of sample types.

HDAC Activity Fluorometric Assay Kit

K2031-100 100 assays
EUR 502

HDAC Activity Colorimetric Assay Kit

K2032-100 100 assays
EUR 502

HAT Activity Colorimetric Assay Kit

K2033-100 100 assays
EUR 557

HAT Activity Fluorometric Assay Kit

K2034-100 100 assays
EUR 557

Calpain Activity Fluorometric Assay Kit

K2062-100 100 assays
EUR 627

KinaseSTAR JNK Activity Assay Kit

K2079-40 40 assays
EUR 738

KinaseSTAR Akt Activity Assay Kit

K2080-40 40 assays
EUR 738

PLTP Activity Fluorometric Assay Kit

K2087-100 100 assays
EUR 669

CETP Activity Fluorometric Assay Kit

K2089-100 100 assays
EUR 725

Proteasome Activity Fluorometric Assay Kit

K2096-100 100 assays
EUR 502

GST Fluorometric Activity Assay Kit

K2105-100 100 assays
EUR 502

GST Colorimetric Activity Assay Kit

K2108-100 100 assays
EUR 529

Trypsin Activity Colorimetric Assay Kit

K2176-100 100 assays
EUR 447

DPP4 Activity Fluorometric Assay Kit

K2178-100 100 assays
EUR 557

Amylase Activity Colorimetric Assay Kit

K2225-100 100 assays
EUR 390

Aconitase Activity Colorimetric Assay Kit

K2226-100 100 assays
EUR 516

Lipase Activity Colorimetric Assay Kit

K2227-100 100 assays
EUR 516

Neuraminidase Activity Fluorometric Assay Kit

K2230-100 100 assays
EUR 599

MAT Activity Assay 3

IK00403 48 tests
EUR 503
Description: Methionine Adenosyltransferase (MAT) activity assay kit

Beta-Mannosidase Activity Assay Kit (Fluorometric)

K2045-100 100 assays
EUR 551

Glucose Isomerase Activity Assay Kit (Colorimetric)

K491-100
EUR 490

Diamine Oxidase Activity Assay Kit (Fluorometric)

K496-100
EUR 566

Oxalate Oxidase Activity Assay Kit (Fluorometric)

K509-100
EUR 479

Mitochondrial Complex III Activity Assay Kit

K520-100
EUR 697

Factor XIIIa Activity Assay Kit (Colorimetric)

K522-100
EUR 620

?-L-Fucosidase Activity Assay Kit (Fluorometric)

K542-100
EUR 523

Cyclooxygenase (COX) Activity Assay Kit (Fluorometric)

K549-100
EUR 419

Glyoxalase I Activity Assay Kit (Colorimetric)

K591-100
EUR 490

PLTP Activity Fluorometric Assay Kit II

K593-100
EUR 588

CETP Activity Fluorometric Assay Kit II

K595-100
EUR 642

Malate Dehydrogenase Activity Colorimetric Assay Kit

K654-100
EUR 572

Asparaginase Activity Colorimetric/Fluorometric Assay Kit

K754-100
EUR 550

Glutathione Reductase Activity Colorimetric Assay Kit

K761-200
EUR 512

Glutathione Peroxidase Activity Colorimetric Assay Kit

K762-100
EUR 512

Thioredoxin Reductase Activity Colorimetric Assay Kit

K763-100
EUR 490

PAF Acetylhydrolase Activity Assay Kit (Colorimetric)

K765-100
EUR 446

PicoProbe? Hexokinase Activity Assay Kit (Fluorometric)

K769-100
EUR 637

Peroxidase Activity Colorimetric/Fluorometric Assay Kit

K772-100
EUR 387

Catalase Activity Colorimetric/Fluorometric Assay Kit

K773-100
EUR 419

Phosphoglucose Isomerase Activity Colorimetric Assay Kit

K775-100
EUR 523

Phosphofructokinase (PFK) Activity Colorimetric Assay Kit

K776-100
EUR 784

MMP-3 Activity Fluorometric Assay Kit

K783-100
EUR 512

Glucose Dehydrogenase Activity Colorimetric Assay Kit

K786-100
EUR 441

Alcohol Dehydrogenase Activity Colorimetric Assay Kit

K787-100
EUR 550

Beta-Lactamase Activity Colorimetric Assay Kit

K803-100
EUR 784

Rat Renin Activity Fluorometric Assay Kit

K806-100
EUR 648

Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit

K807-100
EUR 865

TEV Protease Activity Assay Kit (Fluorometric)

K842-100
EUR 501

p53 Transcription Factor Activity Assay Kit

K923-100
EUR 533

Total Phosphodiesterase Activity Assay Kit (Fluorometric)

K927-100
EUR 615

Lysyl Oxidase Activity Assay Kit (Fluorometric)

K928-100
EUR 533

Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric)

K935-100
EUR 566

PicoProbeTM Glucokinase Activity Assay Kit (Fluorometric)

K969-100
EUR 593

Factor XIa Activity Assay Kit (Colorimetric)

K973-100
EUR 615

Aromatase (CYP19A) Activity Assay Kit (Fluorometric)

K983-100
EUR 620

Alkaline Sphingomyelinase Activity Assay Kit (Colorimetric)

K987-100
EUR 588

5?-Nucleotidase Activity Assay Kit (Colorimetric)

K992-100
EUR 664

Factor XIIa Activity Assay Kit (Colorimetric)

K994-100
EUR 626
For instance, crops are modified to particularly produce molecules with therapeutic makes use of, or with an improved biomass conversion effectivity, or producing bigger volumes of feedstocks for biofuels. Various plant breeding applied sciences are actually utilized in your entire spectrum of plant biotechnology: transgenesis producing proteins or RNAi. Cisgenesis (transgenes remoted from a crossable donor plant) and intragenesis (transgenes originate from the identical species or a crossable species), null segregants are additionally used. To date, the following technology precision gene enhancing instruments are developed in primary analysis. They embody: clustered recurrently interspaced brief palindromic repeats (CRISPR), oligonucleotide-directed mutagenesis (ODM), transcription activator-like results nucleases (TALENs) and zinc-finger nuclease (ZFN).

 

Additive Biotech-Chances, challenges, and recent applications of additive manufacturing technologies in biotechnology.

Additive Biotech-Chances, challenges, and recent applications of additive manufacturing technologies in biotechnology.

The variety and complexity of biotechnological applications are continually rising, with ever increasing ranges of manufacturing hosts, cultivation situations and measurement duties. Consequently, many analytical and cultivation programs for biotechnology and bioprocess engineering, similar to microfluidic units or bioreactors, are tailored to exactly fulfill the necessities of particular measurements or cultivation duties. Additive manufacturing (AM) technologies supply the likelihood of fabricating tailored 3D laboratory tools instantly from CAD designs with beforehand inaccessible ranges of freedom in phrases of structural complexity.

This assessment discusses the historic background of these technologies, their most promising present implementations and the related workflows, fabrication processes and materials specs, along with some of the key challenges related to utilizing AM in biotechnology/bioprocess engineering. To illustrate the good potential of AM, chosen examples in microfluidic units, 3D-bioprinting/biofabrication and bioprocess engineering are highlighted.

Cuban advances in biotech have made headlines, notably for the reason that US-Cuba rapprochement and signing of the historic memorandum of understanding between the US Department of Health and Human Services and Cuba’s Ministry of Public Health in June. Some 34 Cuban establishments with 22,000 workers are the spine of a biotech trade that dates to the early 1980s, acquiring novel merchandise which have sparked curiosity amongst potential international companions. While a quantity of these Cuban merchandise are registered in numerous nations, their testing in the USA stays ensnared in the pink tape of embargo legal guidelines that are inclined to make buyers skittish and thus delay, if not curtail, joint analysis and scientific trial applications to the FDA.

Safety Profile of Anticancer and Immune-Modulating Biotech Drugs Used in a Real World Setting in Campania Region (Italy): BIO-Cam Observational Study.

To examine the prevalence of hostile occasions (AEs) in naïve sufferers receiving biotech medication. Design: A potential observational examine. Setting: Onco-hematology, Hepato-gastroenterology, Rheumatology, Dermatology, and Neurology Units in Campania Region (Italy). Participants: 775 sufferers (53.81% feminine) with imply age 56.0 (SD 15.2). The imply follow-up/affected person was 3.48 (95% confidence interval 3.13-3.84). Main consequence measures: We collected all AEs related to biotech medication, together with severe infections and malignancies.

Serious AEs have been outlined in keeping with the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use, scientific security knowledge administration: definitions and requirements for expedited reporting E2A guideline. Results: The majority of the examine inhabitants was enrolled in Onco-hematology and Rheumatology Units and the commonest analysis have been hematological malignancies, adopted by rheumatoid arthritis, colorectal most cancers, breast most cancers, and psoriatic arthritis.

The mostly prescribed biotech medication have been rituximab, bevacizumab, infliximab, trastuzumab, adalimumab, and cetuximab. Out of 775 sufferers, 320 skilled a minimum of one AE. Most of sufferers skilled AEs to cetuximab remedy, rituximab and trastuzumab. Comparing feminine and male inhabitants, our findings highlighted a statistically important distinction in phrases of AEs for adalimumab (35.90% vs. 7.41%, p < 0.001) and etanercept (27.59% vs. 10.00%, p = 0.023). Considering all biotech medication, we noticed a peak for all AEs prevalence at follow-up 91-180 days class. Bevacizumab, brentuximab, rituximab, trastuzumab and cetuximab have been extra generally related to severe hostile occasions; most of these have been probably associated to biotech medication, in keeping with causality evaluation.

Three circumstances of severe infections occurred. Conclusions: The outcomes of our examine demonstrated that almost all of AEs weren’t severe and anticipated. Few circumstances of severe infections occurred, whereas no case of malignancy did. Overall, the protection profile of biotech medication used in our inhabitants was much like these noticed in pivotal trials. Notwithstanding the optimistic outcomes of our examine, some security issues nonetheless stay unresolved. In order to gather extra effectiveness and security knowledge on biotech medication, the gathering and evaluation of actual world knowledge needs to be endorsed in addition to the administration of post-authorization research.

 Additive Biotech-Chances, challenges, and recent applications of additive manufacturing technologies in biotechnology.

What Will Be the Benefits of Biotech Wheat for European Agriculture?

In European nations, wheat occupies the biggest crop space with excessive yielding manufacturing. France, a significant producer and exporter in Europe, ranks the fifth producer worldwide. Biotic stresses are European farmers’ main challenges (fungal and viral ailments, and insect pests) adopted by abiotic ones similar to drought and grain protein composition. During the final 40 years, 1136 scientific articles on biotech wheat have been printed by USA adopted by China, Australia, Canada, and European Union with the UK. European analysis focuses on pests and ailments resistances utilizing broadly marker-assisted choice (MAS). Transgenesis is used in fundamental analysis to develop resistance towards some fungi (Fusarium head blight) whereas RNA interference (RNAi) silencing is used towards some fungi and virus.

Luciferase Reporter Gene Assay Kit

Z5030003 1,000 assays
EUR 1100
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

SuperLight Luciferase Reporter Gene Assay Kit

SLLU-01K 1000
EUR 502
Description: Bright bioluminescent reagent system allows rapid quantitation of luciferase reporter gene expression in transfected cells within 2 min. Kit size: 1000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

SuperLight Luciferase Reporter Gene Assay Kit

SLLU-200 200
EUR 200
Description: Bright bioluminescent reagent system allows rapid quantitation of luciferase reporter gene expression in transfected cells within 2 min. Kit size: 200 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

SuperLight Luciferase Reporter Gene Assay Kit

SLLU-500 500
EUR 318
Description: Bright bioluminescent reagent system allows rapid quantitation of luciferase reporter gene expression in transfected cells within 2 min. Kit size: 500 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

SuperLight Dual Luciferase Reporter Gene Assay Kit

SLDL-100 100
EUR 200
Description: Bioluminescent reagent system allows rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells within 10 min. Kit size: 100 tests. Shelf life: 12 months. Shipping: ambient temp; storage: -20°C.

Luciferase Reporter Assay Kit

55R-1540 200 assays
EUR 245
Description: Assay Kit for detection of Luciferase Reporter in the research laboratory

Luciferase Reporter Assay Kit

K801-200
EUR 196

Luciferase Reporter Assay Kit

K2181-200 200 assays
EUR 181

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12518 1 plate
EUR 176

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12519 10 plates
EUR 393

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12520 100 plates
EUR 2616

Dual Luciferase Reporter Assay Kit

DL101-01 100 rxn
EUR 258

Single-Luciferase Reporter Assay Kit

20-abx098133
  • EUR 566.00
  • EUR 472.00
  • 200 rxns
  • 50 rxns

Double-Luciferase Reporter Assay Kit

20-abx098134
  • EUR 754.00
  • EUR 519.00
  • 200 rxns
  • 50 rxns

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12530 1 plate
EUR 219

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12531 10 Plates
EUR 702

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12532 100 plates
EUR 3486

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12535 1 plate
EUR 219

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12536 10 plates
EUR 876

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12537 100 plates
EUR 3486

Single-Luciferase (Renilla) Reporter Assay Kit

20-abx298011
  • EUR 457.00
  • EUR 269.00
  • 200 rxns
  • 50 rxns

TFEB promoter-luciferase reporter

PVT18227 2 ug
EUR 300

AlleleBalanced Luciferase Assay Kit

ABP-PA-ABLA001 100 RXNS Ask for price

AlleleBalanced Luciferase Assay Kit

ABP-PA-ABLA011 1000 RXNS Ask for price

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941

Firefly Luciferase Assay Kit (Lyophilized)

30075-1 150TST
EUR 125
Description: Minimum order quantity: 1 unit of 150TST

Firefly Luciferase Assay Kit (Lyophilized)

30075-2 1000TST
EUR 459
Description: Minimum order quantity: 1 unit of 1000TST

Renilla Luciferase Assay Kit 2.0

30082-1 150AS
EUR 172
Description: Minimum order quantity: 1 unit of 150AS

Renilla Luciferase Assay Kit 2.0

30082-2 1000AS
EUR 594
Description: Minimum order quantity: 1 unit of 1000AS

Renilla Luciferase Assay Kit 2.0

30082-T 50AS
EUR 114
Description: Minimum order quantity: 1 unit of 50AS

Firefly Luciferase Assay Kit 2.0

30085-1 150AS
EUR 125
Description: Minimum order quantity: 1 unit of 150AS

Firefly Luciferase Assay Kit 2.0

30085-2 1000AS
EUR 459
Description: Minimum order quantity: 1 unit of 1000AS

Firefly Luciferase Assay Kit 2.0

30085-T 50AS
EUR 109
Description: Minimum order quantity: 1 unit of 50AS

LucyQ Firefly Luciferase Assay Kit

L0010-010 100 Assays
EUR 273

LucyQ Firefly Luciferase Assay Kit

L0010-100 1000 Assays
EUR 617

LucyQ Gaussia Luciferase Assay Kit

L0011-010 100 Assays
EUR 289

LucyQ Gaussia Luciferase Assay Kit

L0011-100 1000 Assays
EUR 944

LucyQ Renilla Luciferase Assay Kit

L0012-010 100 Assays
EUR 273

LucyQ Renilla Luciferase Assay Kit

L0012-100 1000 Assays
EUR 617

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2132

Firefly & Renilla Luciferase Single Tube Assay Kit

30081-1 150AS
EUR 244
Description: Minimum order quantity: 1 unit of 150AS

Firefly & Renilla Luciferase Single Tube Assay Kit

30081-2 1000AS
EUR 984
Description: Minimum order quantity: 1 unit of 1000AS

Firefly & Renilla Luciferase Single Tube Assay Kit

30081-T 50AS
EUR 155
Description: Minimum order quantity: 1 unit of 50AS

LucyQ Duo-Luciferase(Firefly & Renilla) Assay Kit

L0013-010 100 Assays
EUR 317

LucyQ Duo-Luciferase(Firefly & Renilla) Assay Kit

L0013-100 1000 Assays
EUR 1848

CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent)

CASCL9-100A-KIT 1 Kit
EUR 1132

Minicircle Single Reporter: CMV-Luciferase Minicircle (30 ug)

BLIV510MC-1 30 ug
EUR 716

Minicircle Single Reporter: EF1 Luciferase DNA (30 ug)

BLIV511MC-1 30 ug
EUR 716

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

2C::tdTomato Reporter

PVT10473 2 ug
EUR 266

pMIR- Reporter Plasmid

PVT1324 2 ug
EUR 266

Random Hexamers 100 µg

26-4000-03 100 ug
EUR 125
Description:

Random Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers (1,2) and cDNA synthesis. Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. Random Primers can be also used similar to using hexamers in cDNA synthesis in combination with oligo d(T) to yield more 5' end cDNA sequence. Recently random primers have been used to detect DNA polymorphism. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. The authors suggested that these polymorphisms be called RAPD (pronounced RAPID) makers, after Random Amplified Polymorphic DNA (3).

References 1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13. 2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267. 3. Williams J. G., Kubelik A.R., Livak K.J., Rafalski J.A. & Tingey S.V. (1990) Nucleic Acid Res. 18(22):6531-5.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Minicircle Single Reporter: CMV-Luciferase Parental Plasmid (10 ug)

BLIV510MN-1 10 ug
EUR 972

BLIV 2.0 Reporter: CMV-Luciferase-EF1a-copGFP Lentivector Plasmid

BLIV511PA-1 10 ug
EUR 803

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110

Calcium Assay Kit

Z5030014 500 assays
EUR 660
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Chloride Assay Kit

Z5030015 250 assays
EUR 559
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Urea Assay Kit

Z5030016 500 assays
EUR 574
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Phosphate Assay Kit

Z5030017 500 assays
EUR 528
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Creatinine Assay Kit

Z5030020 500 assays
EUR 647
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Iron Assay Kit

Z5030022 250 assays
EUR 677
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

DNA Assay Kit

Z5030023 250 assays
EUR 647
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Copper Assay Kit

Z5030024 250 assays
EUR 947
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Glucose Assay Kit

Z5030025 200 assays
EUR 496
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Hemoglobin Assay Kit

Z5030026 250 assays
EUR 604
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Heme Assay Kit

Z5030027 250 assays
EUR 743
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Ethanol Assay Kit

Z5030029 500 assays
EUR 947
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Glutathione Assay Kit

Z5030030 250 assays
EUR 743
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Magnesium Assay Kit

Z5030032 250 assays
EUR 698
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Peroxide Assay Kit

Z5030034 250 assays
EUR 660
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Sulfate Assay Kit

Z5030035 200 assays
EUR 730
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Zinc Assay Kit

Z5030036 250 assays
EUR 1087
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

ATP Assay Kit

Z5030041 100 assays
EUR 647
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Acetylcholinesterase Assay Kit

Z5030044 100 assays
EUR 838
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Arginase Assay Kit

Z5030047 200 assays
EUR 805
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Lipase Assay Kit

Z5030051 100 assays
EUR 930
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Peroxidase Assay Kit

Z5030052 100 assays
EUR 622
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Bilirubin Assay Kit

Z5030053 180 assays
EUR 677
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Cholesterol Assay Kit

Z5030055 100 assays
EUR 893
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Proteasome Assay Kit

55R-1341 100 assays
EUR 654
Description: Assay Kit for detection of Proteasome in the research laboratory

Calpain Assay Kit

55R-1342 100 assays
EUR 779
Description: Assay Kit for detection of Calpain in the research laboratory

Glutathione Assay Kit

55R-1343 100 assays
EUR 654
Description: Assay Kit for detection of Glutathione in the research laboratory

Glutathione Assay Kit

55R-1354 100 assays
EUR 895
Description: Assay Kit for detection of Glutathione activity in the research laboratory

HDAC Assay Kit

55R-1371 100 assays
EUR 673
Description: Assay Kit for detection of HDAC in the research laboratory

HDAC Assay Kit

55R-1372 100 assays
EUR 673
Description: Assay Kit for detection of HDAC in the research laboratory

HAT Assay Kit

55R-1373 100 assays
EUR 740
Description: Assay Kit for detection of HAT in the research laboratory

SOD Assay Kit

55R-1374 100 assays
EUR 602
Description: Assay Kit for detection of SOD in the research laboratory

HDAC3 Assay Kit

55R-1377 100 assays
EUR 693
Description: Assay Kit for detection of HDAC3 in the research laboratory

HDAC8 Assay Kit

55R-1379 100 assays
EUR 689
Description: Assay Kit for detection of HDAC8 in the research laboratory

ATP Assay Kit

55R-1380 100 assays
EUR 809
Description: Assay Kit for detection of ATP in the research laboratory

ADP Assay Kit

55R-1381 100 assays
EUR 809
Description: Assay Kit for detection of ADP in the research laboratory

FAD Assay Kit

55R-1382 100 assays
EUR 732
Description: Assay Kit for detection of FAD in the research laboratory

PEP Assay Kit

55R-1384 100 assays
EUR 876
Description: Assay Kit for detection of PEP in the research laboratory

Ammonia Assay Kit

55R-1388 100 assays
EUR 740
Description: Assay Kit for detection of Ammonia in the research laboratory

cAMP Assay Kit

55R-1389 100 assays
EUR 706
Description: Assay Kit for detection of cAMP activity in the research laboratory

cGMP Assay Kit

55R-1390 100 assays
EUR 706
Description: Assay Kit for detection of cGMP activity in the research laboratory

Urea Assay Kit

55R-1391 100 assays
EUR 740
Description: Assay Kit for detection of Urea in the research laboratory

Calcium Assay Kit

55R-1392 250 assays
EUR 586
Description: Assay Kit for detection of Calcium activity in the research laboratory

Magnesium Assay Kit

55R-1393 100 assays
EUR 654
Description: Assay Kit for detection of Magnesium in the research laboratory

Zinc Assay Kit

55R-1394 100 assays
EUR 673
Description: Assay Kit for detection of Zinc in the research laboratory

Iron Assay Kit

55R-1395 100 assays
EUR 758
Description: Assay Kit for detection of Iron in the research laboratory

Phosphate Assay Kit

55R-1400 500 assays
EUR 415
Description: Assay Kit for detection of Phosphate in the research laboratory

JNK Assay Kit

55R-1407 40 assays
EUR 868
Description: Assay Kit for detection of JNK in the research laboratory

JNK Assay Kit

55R-1408 40 assays
EUR 979
Description: Assay Kit for detection of JNK in the research laboratory

Akt Assay Kit

55R-1409 40 assays
EUR 979
Description: Assay Kit for detection of Akt in the research laboratory

Ammonia Assay Kit

55R-1410 100 assays
EUR 740
Description: Assay Kit for detection of Ammonia in the research laboratory

Cobalt Assay Kit

55R-1435 100 assays
EUR 621
Description: Assay Kit for detection of Cobalt in the research laboratory

Nickel Assay Kit

55R-1436 100 assays
EUR 621
Description: Assay Kit for detection of Nickel in the research laboratory

Chloride Assay Kit

55R-1437 100 assays
EUR 484
Description: Assay Kit for detection of Chloride in the research laboratory

Aspartate Assay Kit

55R-1438 100 assays
EUR 673
Description: Assay Kit for detection of Aspartate in the research laboratory

Hydroxyproline Assay Kit

55R-1439 100 assays
EUR 844
Description: Assay Kit for detection of Hydroxyproline in the research laboratory

Phenylalanine Assay Kit

55R-1442 100 assays
EUR 654
Description: Assay Kit for detection of Phenylalanine in the research laboratory

Phosphatidylcholine Assay Kit

55R-1443 100 assays
EUR 673
Description: Assay Kit for detection of Phosphatidylcholine in the research laboratory

Glucose Assay Kit

55R-1449 100 assays
EUR 706
Description: Assay Kit for detection of Glucose in the research laboratory

Lactate Assay Kit

55R-1450 100 assays
EUR 844
Description: Assay Kit for detection of Lactate in the research laboratory

Pyruvate Assay Kit

55R-1452 100 assays
EUR 844
Description: Assay Kit for detection of Pyruvate in the research laboratory

Adipogenesis Assay Kit

55R-1454 100 assays
EUR 586
Description: Assay Kit for detection of Adipogenesis in the research laboratory

Glucose Assay Kit

55R-1458 100 assays
EUR 740
Description: Assay Kit for detection of Glucose in the research laboratory

Galactose Assay Kit

55R-1459 100 assays
EUR 740
Description: Assay Kit for detection of Galactose in the research laboratory

Maltose Assay Kit

55R-1460 100 assays
EUR 723
Description: Assay Kit for detection of Maltose in the research laboratory

Fructose Assay Kit

55R-1461 100 assays
EUR 689
Description: Assay Kit for detection of Fructose in the research laboratory

Ethanol Assay Kit

55R-1462 100 assays
EUR 774
Description: Assay Kit for detection of Ethanol in the research laboratory

Galactose Assay Kit

55R-1463 100 assays
EUR 706
Description: Assay Kit for detection of Galactose in the research laboratory

Lactose Assay Kit

55R-1466 100 assays
EUR 706
Description: Assay Kit for detection of Lactose in the research laboratory

Creatinine Assay Kit

55R-1467 100 assays
EUR 638
Description: Assay Kit for detection of Creatinine in the research laboratory

Sucrose Assay Kit

55R-1468 100 assays
EUR 689
Description: Assay Kit for detection of Sucrose in the research laboratory

Lactate Assay Kit

55R-1469 100 assays
EUR 844
Description: Assay Kit for detection of Lactate in the research laboratory

Maltose Assay Kit

55R-1470 100 assays
EUR 706
Description: Assay Kit for detection of Maltose in the research laboratory

Glutamate Assay Kit

55R-1471 100 assays
EUR 740
Description: Assay Kit for detection of Glutamate in the research laboratory

Fumarate Assay Kit

55R-1475 100 assays
EUR 706
Description: Assay Kit for detection of Fumarate in the research laboratory

Creatine Assay Kit

55R-1476 100 assays
EUR 654
Description: Assay Kit for detection of Creatine in the research laboratory

Sarcosine Assay Kit

55R-1477 100 assays
EUR 654
Description: Assay Kit for detection of Sarcosine in the research laboratory

Malate Assay Kit

55R-1478 100 assays
EUR 673
Description: Assay Kit for detection of Malate in the research laboratory

Glycogen Assay Kit

55R-1481 100 assays
EUR 844
Description: Assay Kit for detection of Glycogen in the research laboratory

Starch Assay Kit

55R-1482 100 assays
EUR 673
Description: Assay Kit for detection of Starch in the research laboratory

Alanine Assay Kit

55R-1483 100 assays
EUR 654
Description: Assay Kit for detection of Alanine in the research laboratory

Formate Assay Kit

55R-1484 100 assays
EUR 809
Description: Assay Kit for detection of Formate in the research laboratory

Citrate Assay Kit

55R-1485 100 assays
EUR 740
Description: Assay Kit for detection of Citrate in the research laboratory

Isocitrate Assay Kit

55R-1486 100 assays
EUR 663
Description: Assay Kit for detection of Isocitrate in the research laboratory

Oxaloacetate Assay Kit

55R-1488 100 assays
EUR 663
Description: Assay Kit for detection of Oxaloacetate in the research laboratory

Transgenic crops have been additionally remodeled with genes from numerous species for drought tolerance. The UK (principally with transgenesis and site-specific nucleases) and France (with no transgenic instruments however with MAS and site-specific nucleases) are the primary nations finishing up analysis applications for each biotic stress and drought tolerance. Thus, few European nations used transgenesis for gluten protein composition and RNAi-mediated silencing in celiac illness. Because of vandalism discipline trials of transgenics dropped since 2000. No transgenic wheat is cultivated in Europe for political causes.