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Chemistry, sustainability and naturality of perfumery biotech ingredients

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Chemistry, sustainability and naturality of perfumery biotech ingredients

White biotechnology has emerged in biochemical manufacturing processes to ship perfumery ingredients satisfying novel pursuits of the society for pure, eco-responsible and sustainable supplies. As a consequence, an intense R&D exercise has taken place on these topics, leading to each scientific publications and patent functions reporting combos of state-of-the-art approaches in biocatalysis, metabolic engineering, artificial biology, biosynthesis elucidation, gene version and cloning, and analytical chemistry.

In this minireview, we current a smelly choice of novel biotechnological processes and ingredients from a scientific articles and patents survey protecting the final 6 years and analysed in phrases of chemistry, sustainability and naturalitysynthesis of important oil surrogates or single molecule ingredients, and on one other aspect the optimisation of properties of pure advanced substances by particular and selective enzymatic modifications of their chemical composition. Data had been confirmed, even evaluating luggage based on filling quantity. Data introduced on this research help the conclusion that CryoMACS freezing luggage are bioequivalent to Maco Biotech Freezing-EVA Bags for HPC cryopreservation.

The growing discoveries concerning the biology and capabilities of platelets within the final decade undoubtedly present that these cells are one of probably the most biotechnological human cells. This evaluate summarizes new advances in platelet biology, capabilities, and new ideas of biotech-educated platelets that join superior biomimetic science to platelet-based additive manufacturing for tissue regeneration. As extremely responsive and secretory cells, platelets may very well be explored to develop options that alter injured microenvironments by platelet-based artificial biomaterials with instructive extracellular cues for morphogenesis in tissue engineering past tissue regeneration 2.0.

Comparison of CryoMACS Freezing Bags with Maco Biotech Freezing-Ethinyl Vinyl Acetate Bags for Hematopoietic Progenitor Cells Cryopreservation Using a CD34 +-Enriched Product

Hematopoietic progenitor cells (HPCs) cryopreservation have functions, particularly within the autologous setting, permitting therapeutic use a number of years after assortment. Cryopreservation goals to protect the therapeutic properties of HPCs, and profitable cryopreservation will depend on a number of elements akin to preservation procedures, biopreservation media, freezing charges, and thawing procedures. In this context, the selection of the freezing bag is important because it offers mechanical safety through the freezing course of. Since Maco Biotech Freezing-ethinyl vinyl acetate (EVA) Bags® are now not obtainable in our nation, a comparative research was developed to confirm bioequivalence with the Miltenyi CryoMACS® freezing bag.

In this research, a CD34+-enriched product was used to higher reproduce HPC apheresis. Freezing luggage had been crammed with the identical quantity, cryopreserved with managed price freezing, and saved within the vapor part of liquid nitrogen for no less than 6 months. After thawing, all luggage had been examined for . Classification has been made between metabolic engineering on one aspect, permitting both biotechnological  integrity and sterility utilizing a microbial problem. In addition, a comparability was developed by evaluating restoration of white blood cells, mononuclear cells, lymphocytes, and CD34+ cells.No important variations between the 2 producers’ luggage have been noticed in phrases of the evaluated parameters.

This mini-review offers a perspective of conventional, rising and future functions of lactic acid micro organism (LAB) and how genome modifying instruments can be utilized to beat present challenges in all these functions. It additionally describes obtainable instruments and how these might be additional developed, and takes present laws under consideration. Genome modifying instruments are obligatory for the development of strains for brand spanking new functions and merchandise, however may play an important function in conventional ones, akin to meals and probiotics, as a analysis software for gaining mechanistic insights and discovering new properties.

Traditionally, recombinant DNA methods for LAB have strongly centered on being food-grade, however they lack velocity and the quantity of genetically tractable strains continues to be reasonably restricted. Further software growth will allow fast building of a number of mutants or mutant libraries on a genomic degree in all kinds of LAB strains. We additionally suggest an iterative Design-Build-Test-Learn workflow cycle for LAB cell manufacturing facility growth based mostly on techniques biology, with ‘cell manufacturing facility’ increasing past its conventional which means of manufacturing strains and making use of genome modifying instruments to advance LAB understanding, functions and pressure growth.

Chemistry, sustainability and naturality of perfumery biotech ingredients

Microbe Profile: Komagataella phaffii: a methanol devouring biotech yeast previously generally known as Pichia pastoris

Methylotrophic yeasts of the genus Komagataella are abundantly present in tree exudates. Their potential to make the most of methanol as carbon and power supply depends on an assimilation pathway localized in largely expanded peroxisomes, and a cytosolic methanol dissimilation pathway. Other substrates like glucose or glycerol are readily utilized as effectively. Upon mating diploid cells sporulate readily, forming asci with 4 haploid spores. Their potential to secrete excessive quantities of heterologous proteins made them attention-grabbing for biotechnology, which expands as we speak additionally to different merchandise of major and secondary metabolism.

Polystyrene-bottomed 96-well plate (ELISA)
CT361 6
EUR 73

Adapter for 96 well plate
3510147 1unit
EUR 5581

Streptavidin coated 96-well Plate
6523-5
EUR 327

Black 96 Well Plate, 1EA
X025-1EA 1EA
EUR 73

96 well filter plate (960ul each well)
SD5006 12UNIT
EUR 96.54
  • Product category: Labware/Filtration Supplies/Filter Plates - DNA

96 WELL CLEAR PCR PLATE FOR ABI
PCR-96-AB-C 10/pk
EUR 167
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY
P-DW-12-HC 5/pk
EUR 152
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

Clear Coated 96 Well Plate, 1EA
C107-1EA 1EA
EUR 189

96 well filter plate (no filter)
SD5006-EMPTY 12UNIT
EUR 87.41
  • Product category: Labware/Filtration Supplies/Filter Plates - DNA

Equine Immunoglobulin G (IgG) ELISA Kit
DLR-IgG-Eq-48T 48T
EUR 360
  • Should the Equine Immunoglobulin G (IgG) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Immunoglobulin G (IgG) ELISA Kit
DLR-IgG-Eq-96T 96T
EUR 457
  • Should the Equine Immunoglobulin G (IgG) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Immunoglobulin G (IgG) ELISA Kit
RDR-IgG-Eq-48Tests 48 Tests
EUR 358

Equine Immunoglobulin G (IgG) ELISA Kit
RDR-IgG-Eq-96Tests 96 Tests
EUR 490

96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE.
CP-AXYGEM-96-50 10/pk
EUR 457
Description: Crystallography Plates; Crystallography Plates - Axygen

96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE
PCR-96-HS-C 10/pk
EUR 159
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 well DNA plate with membrane (960ul each well)
SD5007 12UNIT
EUR 242.7
  • Product category: Labware/Filtration Supplies/Filter Plates - DNA

96 well RNA plate with membrane (960ul each well)
SD5009 12UNIT
EUR 260.97
  • Product category: Labware/Filtration Supplies/Filter Plates - RNA

96 Well Breast Tumor Genomic DNA Plate
D8235086-1 1 plate
EUR 316

96 Well Colon Tumor Genomic DNA Plate
D8235090-1 1 plate
EUR 316

96 Well Lung Tumor Genomic DNA Plate
D8235152-1 1 plate
EUR 316

96 Well Stomach Tumor Genomic DNA Plate
D8235248-1 1 plate
EUR 316

Polystyrene-bottomed 96-well plate + lid (ELISPOT)
CT365 5
EUR 78

2 x Clear 96 well Plate, 2EA
X003-2EA 2EA
EUR 65

5 x Clear 96 well Plate, 5EA
X003-5EA 5EA
EUR 80

2 x Clear 96 well Plate, 2EA
X018-2EA 2EA
EUR 65

4 x Clear 96 Well Plate, 4EA
X018-4EA 4EA
EUR 87

Black Half Area 96 Well Plate, 1EA
X023-1EA 1EA
EUR 80

96-Well Plate PCR Products Purification Kit
BS365 5XPlates, 480prep
EUR 250.31
  • Product category: Molecular Biology Kits/DNA - Extraction/PCR Products

96-Well Plate PCR Products Purification Kit
BS3652 2xPlates, 192prep
EUR 155.16
  • Product category: Molecular Biology Kits/DNA - Extraction/PCR Products

96-Well Plate DNA Cleanup Miniprep Kit
BS369 5XPlates, 480prep
EUR 250.31
  • Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

96-Well Plate DNA Cleanup Miniprep Kit
BS3692 2xPlates, 192prep
EUR 155.16
  • Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

96-Well Plate Plasmid DNA Miniprep Kit
BS415 5XPlates, 480prep
EUR 250.31
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

96-Well Plate Plasmid DNA Miniprep Kit
BS4152 2XPlates, 192prep
EUR 155.16
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

96-Well Plate Viral DNA Miniprep Kit
VT92032 2XPlates, 192prep
EUR 413.22
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral

Radius 96-Well Cell Migration Assay
CBA-126 96 assays
EUR 572
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay
CBA-126-5 5 x 96 assays
EUR 2248
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 96-well Cell Transformation Assay
CBA-130 96 assays
EUR 722
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay
CBA-130-5 5 x 96 assays
EUR 2886
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Phagocytosis Assay (Zymosan)
CBA-224 96 assays
EUR 722
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-well Phagocytosis Assay (Zymosan)
CBA-224-5 5 x 96 assays
EUR 2938
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

OxiSelect 96-Well Comet Assay Kit
STA-355 96 assays
EUR 427
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Kit
STA-355-5 5 x 96 assays
EUR 1633
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Slide
STA-356 1 slide
EUR 183
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect 96-Well Comet Assay Slide
STA-356-5 5 slides
EUR 519
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

96-Well ROCK Activity Assay Kit
STA-416 96 assays
EUR 856
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96-Well ROCK Activity Assay Kit
STA-416-5 5 x 96 assays
EUR 3524
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED
P-DW-12-HC-S 5/pk
EUR 207
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included)
ACT-SL-96
EUR 647

Block for 96 well plate, WSC-2620/30
4002659 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

PVDF membrane-bottomed 96-well plate + lid (ELISPOT)
CT366 5
EUR 224

96-well Plasmid DNA Extraction Kit (1 plate)
FAPWE-96001 1 prep
EUR 146

96 Well Plate RNA Cleanup and Concentration Kit
BRC1251 2xPlates, 192prep
EUR 250.31
  • Product category: Molecular Biology Kits/RNA - Extraction/Clean-up

96-Well Plate Animal Genomic DNA Miniprep Kit
BS437 5XPlates, 480prep
EUR 362.98
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

96-Well Plate Animal Genomic DNA Miniprep Kit
BS4372 2XPlates, 192prep
EUR 195.5
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

96 Well Plate Yeast Genomic DNA Miniprep Kit
BS8357 2XPlates, 192prep
EUR 195.5
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Yeast

96-Well Plate Plant Genomic DNA Miniprep Kit
BS8361 2XPlates, 192prep
EUR 195.5
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Plant

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92031 2Plates, 192prep
EUR 175.71
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92032 5Plates, 480prep
EUR 324.92
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1292 2XPlates, 192prep
EUR 175.71
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1295 5XPlates, 480prep
EUR 324.92
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)
EXOEL-96P-1 10 Plates
EUR 213

CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric)
CBA-081 96 assays
EUR 606
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.

96-well Checkpoint Kinase Activity Assay Kit
STA-414 96 assays
EUR 757
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-well Checkpoint Kinase Activity Assay Kit
STA-414-5 5 x 96 assays
EUR 3060
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-Well Assay Plates, Non-Treated, Clear, Round Bottom 1 Plate/Pack, 100/Unit
25-224 100 plates
EUR 299
Description:

Suitable for cell culture but only for suspension cells

  • Optically clear with low autofluorescence for distortion free microscopy
  • Low evaporation lid reduces evaporation and prevents contamination
  • Exterior stacking ring on lid for added stability when stacking
  • Certified RNase & DNase-Free, Non-Pyrogenic & Non-Cytotoxic
  • Sterilized by Gamma Irradiation to a Sterility Assurance Level of 10^-10

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTA55-5P 1 PK
EUR 263

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTG65-5P 1 PK
EUR 263

CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (96 assays)
CBA-320 96 assays
EUR 537
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED
480130 1/pk
EUR 223
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES
480131 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED
480132 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE
432071 1/pk
EUR 345
Description: BioCision; BioCision CoolBox

Block for 96 deep-well plate, WSC-2620/30
4002661 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

Adaptor for 96-well PCR plate, accessory for AgileSealer?
APS-200-96PCR
EUR 351

Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA
C075-1EA 1EA
EUR 178

Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA
C223-1EA 1EA
EUR 178

Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA
C140-1EA 1EA
EUR 178

96 Well PCR Plates, Fits ABI Fast Plate Machines
GP615 25 plates
EUR 212

Goat anti-Mouse, Coated White 96 Well Plate, 1EA
X010-1EA 1EA
EUR 109

Goat anti-Rabbit, Coated White 96 Well Plate, 1EA
X014-1EA 1EA
EUR 109

Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA
X016-1EA 1EA
EUR 109

2 x Black Half Area 96 Well Plate, 2EA
X037-2EA 2EA
EUR 94

Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA
X061-1EA 1EA
EUR 109

Donkey anti-Sheep, Coated White 96 Well Plate, 1EA
X063-1EA 1EA
EUR 109

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS585 5XPlates, 480prep
EUR 487.82
  • Product category: Molecular Biology Kits/RNA - Extraction/Bacteria

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS5852 2XPlates, 192prep
EUR 250.31
  • Product category: Molecular Biology Kits/RNA - Extraction/Bacteria

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92028 2XPlates, 192prep
EUR 212.25
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92029 5XPlates, 480prep
EUR 364.5
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML
PCR-96-AB-C-PBC 10/pk
EUR 195
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

DH10B Chemically Competent Cells (ig10B) - 1x96 well plate - 20µl/well
1018-96 1/EA
EUR 518

CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric
CBA-112-COL 96 assays
EUR 757
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric
CBA-112-LN 96 assays
EUR 757
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Phagocytosis Assay (Red Blood Cell)
CBA-220 96 assays
EUR 635
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

96-well Cellular Senescence Assay (SA ?-Gal Activity)
CBA-231 120 assays
EUR 635
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

96-well Cellular Senescence Assay (SA ?-Gal Activity)
CBA-231-5 5 x 120 assays
EUR 2648
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

Clear 384 Well Plate
X105-1EA 1EA
EUR 112

Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTAG75-5P 1 PK
EUR 286

Goat anti-Mouse, 1 x Whole Well, Clear Coated 96 Well Plate, 1EA
X011-1EA 1EA
EUR 102

FALCON® 96 WELL CLEAR ROUND BOTTOM NOT TREATED ASSAY PLATE, NONSTERILE, 5/PACK, 50/CASE
353910 5/pk
EUR 91
Description: Assay - DL; Assay 96 well - DL

DNA-BIND™ PLATE, 96 WELL, CLEAR, FLAT 1/10
2525 1/pk
EUR 137
Description: Genomics; Genomic Plates

Mouse anti-Cystatin C Clear Coated 96 Well Plate, 1EA
C033-1EA 1EA
EUR 178

Mouse anti-Endothelin-1 Clear Coated 96 Well Plate, 1EA
C161-1EA 1EA
EUR 178

96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based)
B814152-0002 2XPlates, 2XPlates
EUR 155.16
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based)
B814152-0005 5XPlates, 5XPlates
EUR 250.31
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

96 Well 0.2ml PCR Plate, Chimney-Top 10/Bag, Sterile
PCR-960-P-CH-S 1PK, 10UNIT
EUR 92.79
  • Product category: Labware/Multiwell Plates/96-Well

Sealing film, PCR 96 Well Plate Sealing Membrane, 100/pk
P1001-PCR 100/pack
EUR 95.9
Description: Sealing film PCR 96 Well Plate Sealing Membrane

CytoSelect 96-well Cell Migration Assay (3 ?m), Fluorometric
CBA-104 96 assays
EUR 635
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 96-well Cell Migration Assay (3 ?m), Fluorometric
CBA-104-5 5 x 96 assays
EUR 2613
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 96-well Cell Migration Assay (5 ?m), Fluorometric
CBA-105 96 assays
EUR 635
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 96-well Cell Migration Assay (5 ?m), Fluorometric
CBA-105-5 5 x 96 assays
EUR 2613
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 96-well Cell Migration Assay (8 ?m), Fluorometric
CBA-106 96 assays
EUR 635
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 96-well Cell Migration Assay (8 ?m), Fluorometric
CBA-106-5 5 x 96 assays
EUR 2613
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 96-Well Phagocytosis Assay (E. coli, Colorimetric Format)
CBA-222 96 assays
EUR 693
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, E. coli Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-Well Phagocytosis Assay(Zymosan Substrate), Trial Size
CBA-224-T 20 assays
EUR 386
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

EZCellTM Cell Invasion Assay (laminin), 96-well, 8 µm
K914-100
EUR 566

Goat anti-Mouse, 1 x 8 Strip Well, Clear Coated 96 Well Plate, 1EA
X012-1EA 1EA
EUR 109

96 WELL CLEAR PCR MICROPLATE
PCR-96-C 10/pk
EUR 145
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (5 x 96 assays)
CBA-320-5 5 x 96 assays
EUR 2114
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

96 WELL BARCODED H1-H12, CLEAR PCR FULL SKIRT PCR AMPLIFICATION MICRO PLATE
PCR-96-FS-C-PBC 10/pk
EUR 168
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96-Well CometSlides
4253-096-03 1 Tests
EUR 142.4

Adapter for 24 well plate
3510146 1unit
EUR 5581

Random Nanofibers 24 Well Plate
3D00006-24 700 nm-PCLs
EUR 101

Targeted genome modifying is a sophisticated method that allows exact modification of the nucleic acid sequences in a genome. Genome modifying is usually carried out utilizing instruments, akin to molecular scissors, to chop an outlined location in a selected gene. Genome modifying has impacted numerous fields of biotechnology, akin to agriculture; biopharmaceutical manufacturing; research on the construction, regulation, and perform of the genome; and the creation of transgenic organisms and cell strains. Although genome modifying is used often, it has a number of limitations.

Here, we offer an outline of well-studied genome-editing nucleases, together with single-stranded oligodeoxynucleotides (ssODNs), transcription activator-like effector nucleases (TALENs), zinc-finger nucleases (ZFNs), and CRISPR-Cas9 RNA-guided nucleases (CRISPR-Cas9). To this finish, we describe the progress towards editable nuclease-based therapies and talk about the minimization of off-target mutagenesis. Future prospects of this difficult scientific area are additionally mentioned.  Komagataella yeasts normally develop as haploid cells and are secondary homothallic as they will change mating sort.