White biotechnology has emerged in biochemical manufacturing processes to ship perfumery ingredients satisfying novel pursuits of the society for pure, eco-responsible and sustainable supplies. As a consequence, an intense R&D exercise has taken place on these topics, leading to each scientific publications and patent functions reporting combos of state-of-the-art approaches in biocatalysis, metabolic engineering, artificial biology, biosynthesis elucidation, gene version and cloning, and analytical chemistry.
In this minireview, we current a smelly choice of novel biotechnological processes and ingredients from a scientific articles and patents survey protecting the final 6 years and analysed in phrases of chemistry, sustainability and naturalitysynthesis of important oil surrogates or single molecule ingredients, and on one other aspect the optimisation of properties of pure advanced substances by particular and selective enzymatic modifications of their chemical composition. Data had been confirmed, even evaluating luggage based on filling quantity. Data introduced on this research help the conclusion that CryoMACS freezing luggage are bioequivalent to Maco Biotech Freezing-EVA Bags for HPC cryopreservation.
The growing discoveries concerning the biology and capabilities of platelets within the final decade undoubtedly present that these cells are one of probably the most biotechnological human cells. This evaluate summarizes new advances in platelet biology, capabilities, and new ideas of biotech-educated platelets that join superior biomimetic science to platelet-based additive manufacturing for tissue regeneration. As extremely responsive and secretory cells, platelets may very well be explored to develop options that alter injured microenvironments by platelet-based artificial biomaterials with instructive extracellular cues for morphogenesis in tissue engineering past tissue regeneration 2.0.
Comparison of CryoMACS Freezing Bags with Maco Biotech Freezing-Ethinyl Vinyl Acetate Bags for Hematopoietic Progenitor Cells Cryopreservation Using a CD34 +-Enriched Product
Hematopoietic progenitor cells (HPCs) cryopreservation have functions, particularly within the autologous setting, permitting therapeutic use a number of years after assortment. Cryopreservation goals to protect the therapeutic properties of HPCs, and profitable cryopreservation will depend on a number of elements akin to preservation procedures, biopreservation media, freezing charges, and thawing procedures. In this context, the selection of the freezing bag is important because it offers mechanical safety through the freezing course of. Since Maco Biotech Freezing-ethinyl vinyl acetate (EVA) Bags® are now not obtainable in our nation, a comparative research was developed to confirm bioequivalence with the Miltenyi CryoMACS® freezing bag.
In this research, a CD34+-enriched product was used to higher reproduce HPC apheresis. Freezing luggage had been crammed with the identical quantity, cryopreserved with managed price freezing, and saved within the vapor part of liquid nitrogen for no less than 6 months. After thawing, all luggage had been examined for . Classification has been made between metabolic engineering on one aspect, permitting both biotechnological integrity and sterility utilizing a microbial problem. In addition, a comparability was developed by evaluating restoration of white blood cells, mononuclear cells, lymphocytes, and CD34+ cells.No important variations between the 2 producers’ luggage have been noticed in phrases of the evaluated parameters.
This mini-review offers a perspective of conventional, rising and future functions of lactic acid micro organism (LAB) and how genome modifying instruments can be utilized to beat present challenges in all these functions. It additionally describes obtainable instruments and how these might be additional developed, and takes present laws under consideration. Genome modifying instruments are obligatory for the development of strains for brand spanking new functions and merchandise, however may play an important function in conventional ones, akin to meals and probiotics, as a analysis software for gaining mechanistic insights and discovering new properties.
Traditionally, recombinant DNA methods for LAB have strongly centered on being food-grade, however they lack velocity and the quantity of genetically tractable strains continues to be reasonably restricted. Further software growth will allow fast building of a number of mutants or mutant libraries on a genomic degree in all kinds of LAB strains. We additionally suggest an iterative Design-Build-Test-Learn workflow cycle for LAB cell manufacturing facility growth based mostly on techniques biology, with ‘cell manufacturing facility’ increasing past its conventional which means of manufacturing strains and making use of genome modifying instruments to advance LAB understanding, functions and pressure growth.

Microbe Profile: Komagataella phaffii: a methanol devouring biotech yeast previously generally known as Pichia pastoris
Methylotrophic yeasts of the genus Komagataella are abundantly present in tree exudates. Their potential to make the most of methanol as carbon and power supply depends on an assimilation pathway localized in largely expanded peroxisomes, and a cytosolic methanol dissimilation pathway. Other substrates like glucose or glycerol are readily utilized as effectively. Upon mating diploid cells sporulate readily, forming asci with 4 haploid spores. Their potential to secrete excessive quantities of heterologous proteins made them attention-grabbing for biotechnology, which expands as we speak additionally to different merchandise of major and secondary metabolism.
96 well filter plate (960ul each well) |
SD5006 |
Bio Basic |
12UNIT |
EUR 96.54 |
|
96 WELL CLEAR PCR PLATE FOR ABI |
PCR-96-AB-C |
CORNING |
10/pk |
EUR 167 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY |
P-DW-12-HC |
CORNING |
5/pk |
EUR 152 |
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
Clear Coated 96 Well Plate, 1EA |
C107-1EA |
Arbor Assays |
1EA |
EUR 189 |
96 well filter plate (no filter) |
SD5006-EMPTY |
Bio Basic |
12UNIT |
EUR 87.41 |
|
96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE |
PCR-96-HS-C |
CORNING |
10/pk |
EUR 159 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE. |
CP-AXYGEM-96-50 |
CORNING |
10/pk |
EUR 457 |
Description: Crystallography Plates; Crystallography Plates - Axygen |
Equine Immunoglobulin G (IgG) ELISA Kit |
DLR-IgG-Eq-48T |
DL Develop |
48T |
EUR 360 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Equine Immunoglobulin G (IgG) ELISA Kit |
DLR-IgG-Eq-96T |
DL Develop |
96T |
EUR 457 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Equine Immunoglobulin G (IgG) ELISA Kit |
RDR-IgG-Eq-48Tests |
Reddot Biotech |
48 Tests |
EUR 358 |
Equine Immunoglobulin G (IgG) ELISA Kit |
RDR-IgG-Eq-96Tests |
Reddot Biotech |
96 Tests |
EUR 490 |
96 well DNA plate with membrane (960ul each well) |
SD5007 |
Bio Basic |
12UNIT |
EUR 242.7 |
|
96 well RNA plate with membrane (960ul each well) |
SD5009 |
Bio Basic |
12UNIT |
EUR 260.97 |
|
96 Well Breast Tumor Genomic DNA Plate |
D8235086-1 |
Biochain |
1 plate |
EUR 316 |
96 Well Colon Tumor Genomic DNA Plate |
D8235090-1 |
Biochain |
1 plate |
EUR 316 |
96 Well Lung Tumor Genomic DNA Plate |
D8235152-1 |
Biochain |
1 plate |
EUR 316 |
96 Well Stomach Tumor Genomic DNA Plate |
D8235248-1 |
Biochain |
1 plate |
EUR 316 |
2 x Clear 96 well Plate, 2EA |
X003-2EA |
Arbor Assays |
2EA |
EUR 65 |
5 x Clear 96 well Plate, 5EA |
X003-5EA |
Arbor Assays |
5EA |
EUR 80 |
2 x Clear 96 well Plate, 2EA |
X018-2EA |
Arbor Assays |
2EA |
EUR 65 |
4 x Clear 96 Well Plate, 4EA |
X018-4EA |
Arbor Assays |
4EA |
EUR 87 |
Black Half Area 96 Well Plate, 1EA |
X023-1EA |
Arbor Assays |
1EA |
EUR 80 |
96-Well Plate PCR Products Purification Kit |
BS365 |
Bio Basic |
5XPlates, 480prep |
EUR 250.31 |
|
96-Well Plate PCR Products Purification Kit |
BS3652 |
Bio Basic |
2xPlates, 192prep |
EUR 155.16 |
|
96-Well Plate DNA Cleanup Miniprep Kit |
BS369 |
Bio Basic |
5XPlates, 480prep |
EUR 250.31 |
|
96-Well Plate DNA Cleanup Miniprep Kit |
BS3692 |
Bio Basic |
2xPlates, 192prep |
EUR 155.16 |
|
96-Well Plate Plasmid DNA Miniprep Kit |
BS415 |
Bio Basic |
5XPlates, 480prep |
EUR 250.31 |
|
96-Well Plate Plasmid DNA Miniprep Kit |
BS4152 |
Bio Basic |
2XPlates, 192prep |
EUR 155.16 |
|
96-Well Plate Viral DNA Miniprep Kit |
VT92032 |
Bio Basic |
2XPlates, 192prep |
EUR 413.22 |
|
Polystyrene-bottomed 96-well plate + lid (ELISPOT) |
CT365 |
U-CyTech |
5 |
EUR 78 |
Radius 96-Well Cell Migration Assay |
CBA-126 |
Cell Biolabs |
96 assays |
EUR 572 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 96-Well Cell Migration Assay |
CBA-126-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2248 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
CytoSelect 96-well Cell Transformation Assay |
CBA-130 |
Cell Biolabs |
96 assays |
EUR 722 |
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
CytoSelect 96-well Cell Transformation Assay |
CBA-130-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2886 |
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
CytoSelect 96-well Phagocytosis Assay (Zymosan) |
CBA-224 |
Cell Biolabs |
96 assays |
EUR 722 |
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
CytoSelect 96-well Phagocytosis Assay (Zymosan) |
CBA-224-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2938 |
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
OxiSelect 96-Well Comet Assay Kit |
STA-355 |
Cell Biolabs |
96 assays |
EUR 427 |
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
OxiSelect 96-Well Comet Assay Kit |
STA-355-5 |
Cell Biolabs |
5 x 96 assays |
EUR 1633 |
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
OxiSelect 96-Well Comet Assay Slide |
STA-356 |
Cell Biolabs |
1 slide |
EUR 183 |
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
OxiSelect 96-Well Comet Assay Slide |
STA-356-5 |
Cell Biolabs |
5 slides |
EUR 519 |
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
96-Well ROCK Activity Assay Kit |
STA-416 |
Cell Biolabs |
96 assays |
EUR 856 |
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1. |
96-Well ROCK Activity Assay Kit |
STA-416-5 |
Cell Biolabs |
5 x 96 assays |
EUR 3524 |
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1. |
SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included) |
ACT-SL-96 |
ACTGene |
|
EUR 647 |
1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED |
P-DW-12-HC-S |
CORNING |
5/pk |
EUR 207 |
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
96-well Plasmid DNA Extraction Kit (1 plate) |
FAPWE-96001 |
Favorgen |
1 prep |
EUR 146 |
96 Well Plate RNA Cleanup and Concentration Kit |
BRC1251 |
Bio Basic |
2xPlates, 192prep |
EUR 250.31 |
|
96-Well Plate Animal Genomic DNA Miniprep Kit |
BS437 |
Bio Basic |
5XPlates, 480prep |
EUR 362.98 |
|
96-Well Plate Animal Genomic DNA Miniprep Kit |
BS4372 |
Bio Basic |
2XPlates, 192prep |
EUR 195.5 |
|
96 Well Plate Yeast Genomic DNA Miniprep Kit |
BS8357 |
Bio Basic |
2XPlates, 192prep |
EUR 195.5 |
|
96-Well Plate Plant Genomic DNA Miniprep Kit |
BS8361 |
Bio Basic |
2XPlates, 192prep |
EUR 195.5 |
|
96 Well Plate Blood Genomic DNA Miniprep Kit |
BT92031 |
Bio Basic |
2Plates, 192prep |
EUR 175.71 |
|
96 Well Plate Blood Genomic DNA Miniprep Kit |
BT92032 |
Bio Basic |
5Plates, 480prep |
EUR 324.92 |
|
Block for 96 well plate, WSC-2620/30 |
4002659 |
Atto |
1unit |
EUR 382 |
Description: Bl ock for WSC-2620 / 2630 |
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
SK1292 |
Bio Basic |
2XPlates, 192prep |
EUR 175.71 |
|
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
SK1295 |
Bio Basic |
5XPlates, 480prep |
EUR 324.92 |
|
PVDF membrane-bottomed 96-well plate + lid (ELISPOT) |
CT366 |
U-CyTech |
5 |
EUR 224 |
96 Well ExoELISA plate (12x8 well strips, pack of 10 plates) |
EXOEL-96P-1 |
SBI |
10 Plates |
EUR 213 |
CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric) |
CBA-081 |
Cell Biolabs |
96 assays |
EUR 606 |
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included. |
96-well Checkpoint Kinase Activity Assay Kit |
STA-414 |
Cell Biolabs |
96 assays |
EUR 757 |
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors. |
96-well Checkpoint Kinase Activity Assay Kit |
STA-414-5 |
Cell Biolabs |
5 x 96 assays |
EUR 3060 |
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors. |
96-Well Assay Plates, Non-Treated, Clear, Round Bottom 1 Plate/Pack, 100/Unit |
25-224 |
Genesee Scientific |
100 plates |
EUR 299 |
Description: Suitable for cell culture but only for suspension cells - Optically clear with low autofluorescence for distortion free microscopy
- Low evaporation lid reduces evaporation and prevents contamination
- Exterior stacking ring on lid for added stability when stacking
- Certified RNase & DNase-Free, Non-Pyrogenic & Non-Cytotoxic
- Sterilized by Gamma Irradiation to a Sterility Assurance Level of 10^-10
|
DH10B Chemically Competent Cells (ig10B) - 1x96 well plate - 20µl/well |
1018-96 |
Intact Genomics |
1/EA |
EUR 518 |
96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML |
PCR-96-AB-C-PBC |
CORNING |
10/pk |
EUR 195 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
PRTA55-5P |
Alpha Diagnostics |
1 PK |
EUR 263 |
Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
PRTG65-5P |
Alpha Diagnostics |
1 PK |
EUR 263 |
Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA |
C075-1EA |
Arbor Assays |
1EA |
EUR 178 |
Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA |
C140-1EA |
Arbor Assays |
1EA |
EUR 178 |
Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA |
C223-1EA |
Arbor Assays |
1EA |
EUR 178 |
Goat anti-Mouse, Coated White 96 Well Plate, 1EA |
X010-1EA |
Arbor Assays |
1EA |
EUR 109 |
Goat anti-Rabbit, Coated White 96 Well Plate, 1EA |
X014-1EA |
Arbor Assays |
1EA |
EUR 109 |
Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA |
X016-1EA |
Arbor Assays |
1EA |
EUR 109 |
2 x Black Half Area 96 Well Plate, 2EA |
X037-2EA |
Arbor Assays |
2EA |
EUR 94 |
Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA |
X061-1EA |
Arbor Assays |
1EA |
EUR 109 |
Donkey anti-Sheep, Coated White 96 Well Plate, 1EA |
X063-1EA |
Arbor Assays |
1EA |
EUR 109 |
Adaptor for 96-well PCR plate, accessory for AgileSealer? |
APS-200-96PCR |
ACTGene |
|
EUR 351 |
96 Well Plate Bacterial Total RNA Miniprep Super Kit |
BS585 |
Bio Basic |
5XPlates, 480prep |
EUR 487.82 |
|
96 Well Plate Bacterial Total RNA Miniprep Super Kit |
BS5852 |
Bio Basic |
2XPlates, 192prep |
EUR 250.31 |
|
Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit |
BS92028 |
Bio Basic |
2XPlates, 192prep |
EUR 212.25 |
|
Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit |
BS92029 |
Bio Basic |
5XPlates, 480prep |
EUR 364.5 |
|
Block for 96 deep-well plate, WSC-2620/30 |
4002661 |
Atto |
1unit |
EUR 382 |
Description: Bl ock for WSC-2620 / 2630 |
COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE |
432071 |
CORNING |
1/pk |
EUR 345 |
Description: BioCision; BioCision CoolBox |
SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED |
480130 |
CORNING |
1/pk |
EUR 223 |
Description: Lab Equipment; Constant Temperature Equipment |
DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES |
480131 |
CORNING |
1/pk |
EUR 218 |
Description: Lab Equipment; Constant Temperature Equipment |
DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED |
480132 |
CORNING |
1/pk |
EUR 218 |
Description: Lab Equipment; Constant Temperature Equipment |
96 Well PCR Plates, Fits ABI Fast Plate Machines |
GP615 |
GenDepot |
25 plates |
EUR 212 |
CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (96 assays) |
CBA-320 |
Cell Biolabs |
96 assays |
EUR 537 |
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis. |
CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric |
CBA-112-COL |
Cell Biolabs |
96 assays |
EUR 757 |
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I. |
CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric |
CBA-112-LN |
Cell Biolabs |
96 assays |
EUR 757 |
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin. |
CytoSelect 96-well Phagocytosis Assay (Red Blood Cell) |
CBA-220 |
Cell Biolabs |
96 assays |
EUR 635 |
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
96-well Cellular Senescence Assay (SA ?-Gal Activity) |
CBA-231 |
Cell Biolabs |
120 assays |
EUR 635 |
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader. |
96-well Cellular Senescence Assay (SA ?-Gal Activity) |
CBA-231-5 |
Cell Biolabs |
5 x 120 assays |
EUR 2648 |
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader. |
Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
PRTAG75-5P |
Alpha Diagnostics |
1 PK |
EUR 286 |
Goat anti-Mouse, 1 x Whole Well, Clear Coated 96 Well Plate, 1EA |
X011-1EA |
Arbor Assays |
1EA |
EUR 102 |
FALCON® 96 WELL CLEAR ROUND BOTTOM NOT TREATED ASSAY PLATE, NONSTERILE, 5/PACK, 50/CASE |
353910 |
CORNING |
5/pk |
EUR 91 |
Description: Assay - DL; Assay 96 well - DL |
Mouse anti-Cystatin C Clear Coated 96 Well Plate, 1EA |
C033-1EA |
Arbor Assays |
1EA |
EUR 178 |
Mouse anti-Endothelin-1 Clear Coated 96 Well Plate, 1EA |
C161-1EA |
Arbor Assays |
1EA |
EUR 178 |
96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based) |
B814152-0002 |
Bio Basic |
2XPlates, 2XPlates |
EUR 155.16 |
|
96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based) |
B814152-0005 |
Bio Basic |
5XPlates, 5XPlates |
EUR 250.31 |
|
96 Well 0.2ml PCR Plate, Chimney-Top 10/Bag, Sterile |
PCR-960-P-CH-S |
Bio Basic |
1PK, 10UNIT |
EUR 92.79 |
|
DNA-BIND™ PLATE, 96 WELL, CLEAR, FLAT 1/10 |
2525 |
CORNING |
1/pk |
EUR 137 |
Description: Genomics; Genomic Plates |
Sealing film, PCR 96 Well Plate Sealing Membrane, 100/pk |
P1001-PCR |
MTC Bio |
100/pack |
EUR 95.9 |
Description: Sealing film PCR 96 Well Plate Sealing Membrane |
96 WELL CLEAR PCR MICROPLATE |
PCR-96-C |
CORNING |
10/pk |
EUR 145 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
384 Well Plate, 10/Bag |
PCR-384-SN |
Bio Basic |
1PK, 10UNIT |
EUR 93.5 |
|
Adapter for 24 well plate |
3510146 |
Atto |
1unit |
EUR 5581 |
Tissue Culture Plate, 12 Well |
TCP20-12 |
Bio Basic |
1 UNIT |
EUR 53.48 |
|
Tissue Culture Plate, 48 Well |
TCP20-48 |
Bio Basic |
1 UNIT |
EUR 53.48 |
|
Tissue Culture Plate, 6 Well |
TCP20-6 |
Bio Basic |
1 UNIT |
EUR 53.48 |
|
Random Nanofibers 24 Well Plate |
3D00006-24 |
Neuromics |
700 nm-PCLs |
EUR 101 |
Random Nanofibers 348 Well Plate |
3D00006-384 |
Neuromics |
700 nm-PCLs |
EUR 104 |
Random Nanofibers 6 Well Plate |
3D00006-6 |
Neuromics |
700 nm-PCLs |
EUR 93 |
Aligned Nanofibers 24 Well Plate |
3D00012-24 |
Neuromics |
700 nm-PCLs |
EUR 104 |
Aligned Nanofibers 348 Well Plate |
3D00012-384 |
Neuromics |
700 nm-PCLs |
EUR 123 |
Aligned Nanofibers 6 Well Plate |
3D00012-6 |
Neuromics |
700 nm-PCLs |
EUR 97 |
EZCellTM Cell Invasion Assay (laminin), 96-well, 8 µm |
K914-100 |
Biovision |
|
EUR 566 |
CytoSelect 96-well Cell Migration Assay (3 ?m), Fluorometric |
CBA-104 |
Cell Biolabs |
96 assays |
EUR 635 |
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes. |
CytoSelect 96-well Cell Migration Assay (3 ?m), Fluorometric |
CBA-104-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2613 |
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes. |
CytoSelect 96-well Cell Migration Assay (5 ?m), Fluorometric |
CBA-105 |
Cell Biolabs |
96 assays |
EUR 635 |
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages. |
CytoSelect 96-well Cell Migration Assay (5 ?m), Fluorometric |
CBA-105-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2613 |
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages. |
CytoSelect 96-well Cell Migration Assay (8 ?m), Fluorometric |
CBA-106 |
Cell Biolabs |
96 assays |
EUR 635 |
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines. |
CytoSelect 96-well Cell Migration Assay (8 ?m), Fluorometric |
CBA-106-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2613 |
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines. |
Targeted genome modifying is a sophisticated method that allows exact modification of the nucleic acid sequences in a genome. Genome modifying is usually carried out utilizing instruments, akin to molecular scissors, to chop an outlined location in a selected gene. Genome modifying has impacted numerous fields of biotechnology, akin to agriculture; biopharmaceutical manufacturing; research on the construction, regulation, and perform of the genome; and the creation of transgenic organisms and cell strains. Although genome modifying is used often, it has a number of limitations.
Here, we offer an outline of well-studied genome-editing nucleases, together with single-stranded oligodeoxynucleotides (ssODNs), transcription activator-like effector nucleases (TALENs), zinc-finger nucleases (ZFNs), and CRISPR-Cas9 RNA-guided nucleases (CRISPR-Cas9). To this finish, we describe the progress towards editable nuclease-based therapies and talk about the minimization of off-target mutagenesis. Future prospects of this difficult scientific area are additionally mentioned. Komagataella yeasts normally develop as haploid cells and are secondary homothallic as they will change mating sort.