Additive Biotech-Chances, challenges, and recent applications of additive manufacturing technologies in biotechnology.

Additive Biotech-Chances, challenges, and recent applications of additive manufacturing technologies in biotechnology.

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The variety and complexity of biotechnological applications are continually rising, with ever increasing ranges of manufacturing hosts, cultivation situations and measurement duties. Consequently, many analytical and cultivation programs for biotechnology and bioprocess engineering, similar to microfluidic units or bioreactors, are tailored to exactly fulfill the necessities of particular measurements or cultivation duties. Additive manufacturing (AM) technologies supply the likelihood of fabricating tailored 3D laboratory tools instantly from CAD designs with beforehand inaccessible ranges of freedom in phrases of structural complexity.

This assessment discusses the historic background of these technologies, their most promising present implementations and the related workflows, fabrication processes and materials specs, along with some of the key challenges related to utilizing AM in biotechnology/bioprocess engineering. To illustrate the good potential of AM, chosen examples in microfluidic units, 3D-bioprinting/biofabrication and bioprocess engineering are highlighted.

Cuban advances in biotech have made headlines, notably for the reason that US-Cuba rapprochement and signing of the historic memorandum of understanding between the US Department of Health and Human Services and Cuba’s Ministry of Public Health in June. Some 34 Cuban establishments with 22,000 workers are the spine of a biotech trade that dates to the early 1980s, acquiring novel merchandise which have sparked curiosity amongst potential international companions. While a quantity of these Cuban merchandise are registered in numerous nations, their testing in the USA stays ensnared in the pink tape of embargo legal guidelines that are inclined to make buyers skittish and thus delay, if not curtail, joint analysis and scientific trial applications to the FDA.

Safety Profile of Anticancer and Immune-Modulating Biotech Drugs Used in a Real World Setting in Campania Region (Italy): BIO-Cam Observational Study.

To examine the prevalence of hostile occasions (AEs) in naïve sufferers receiving biotech medication. Design: A potential observational examine. Setting: Onco-hematology, Hepato-gastroenterology, Rheumatology, Dermatology, and Neurology Units in Campania Region (Italy). Participants: 775 sufferers (53.81% feminine) with imply age 56.0 (SD 15.2). The imply follow-up/affected person was 3.48 (95% confidence interval 3.13-3.84). Main consequence measures: We collected all AEs related to biotech medication, together with severe infections and malignancies.

Serious AEs have been outlined in keeping with the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use, scientific security knowledge administration: definitions and requirements for expedited reporting E2A guideline. Results: The majority of the examine inhabitants was enrolled in Onco-hematology and Rheumatology Units and the commonest analysis have been hematological malignancies, adopted by rheumatoid arthritis, colorectal most cancers, breast most cancers, and psoriatic arthritis.

The mostly prescribed biotech medication have been rituximab, bevacizumab, infliximab, trastuzumab, adalimumab, and cetuximab. Out of 775 sufferers, 320 skilled a minimum of one AE. Most of sufferers skilled AEs to cetuximab remedy, rituximab and trastuzumab. Comparing feminine and male inhabitants, our findings highlighted a statistically important distinction in phrases of AEs for adalimumab (35.90% vs. 7.41%, p < 0.001) and etanercept (27.59% vs. 10.00%, p = 0.023). Considering all biotech medication, we noticed a peak for all AEs prevalence at follow-up 91-180 days class. Bevacizumab, brentuximab, rituximab, trastuzumab and cetuximab have been extra generally related to severe hostile occasions; most of these have been probably associated to biotech medication, in keeping with causality evaluation.

Three circumstances of severe infections occurred. Conclusions: The outcomes of our examine demonstrated that almost all of AEs weren’t severe and anticipated. Few circumstances of severe infections occurred, whereas no case of malignancy did. Overall, the protection profile of biotech medication used in our inhabitants was much like these noticed in pivotal trials. Notwithstanding the optimistic outcomes of our examine, some security issues nonetheless stay unresolved. In order to gather extra effectiveness and security knowledge on biotech medication, the gathering and evaluation of actual world knowledge needs to be endorsed in addition to the administration of post-authorization research.

Additive Biotech-Chances, challenges, and recent applications of additive manufacturing technologies in biotechnology.

What Will Be the Benefits of Biotech Wheat for European Agriculture?

In European nations, wheat occupies the biggest crop space with excessive yielding manufacturing. France, a significant producer and exporter in Europe, ranks the fifth producer worldwide. Biotic stresses are European farmers’ main challenges (fungal and viral ailments, and insect pests) adopted by abiotic ones similar to drought and grain protein composition. During the final 40 years, 1136 scientific articles on biotech wheat have been printed by USA adopted by China, Australia, Canada, and European Union with the UK. European analysis focuses on pests and ailments resistances utilizing broadly marker-assisted choice (MAS). Transgenesis is used in fundamental analysis to develop resistance towards some fungi (Fusarium head blight) whereas RNA interference (RNAi) silencing is used towards some fungi and virus.

Luciferase Reporter Gene Assay Kit
Z5030003 1,000 assays
EUR 1118

Dual Luciferase Reporter Gene Assay Kit
KTA8010-1000T 1000 T
EUR 819

Dual Luciferase Reporter Gene Assay Kit
KTA8010-100T 100 T
EUR 149

Dual Luciferase Reporter Gene Assay Kit
KTA8010-each each Ask for price

Dual Luciferase Reporter Gene Assay Kit
RG027 100 Tests Ask for price

Dual Luciferase Reporter Gene Assay Kit
RG028 1000 Tests Ask for price

Firefly Luciferase Reporter Gene Assay Kit
RG005 100 Tests Ask for price

Firefly Luciferase Reporter Gene Assay Kit
RG006 1000 Tests Ask for price

Renilla Luciferase Reporter Gene Assay Kit
RG016 100 Tests Ask for price

Renilla Luciferase Reporter Gene Assay Kit
RG017 1000 Tests Ask for price

Gaussia Luciferase Reporter Gene Assay Kit
RG021M 1000 Tests Ask for price

Gaussia Luciferase Reporter Gene Assay Kit
RG021S 100 Tests Ask for price

Dual Luciferase Reporter Gene Assay Kit II
RG029M 1000 Tests Ask for price

Dual Luciferase Reporter Gene Assay Kit II
RG029S 100 Tests Ask for price

Firefly Luciferase Reporter Gene Assay Kit II
RG007M 1000 Tests Ask for price

Firefly Luciferase Reporter Gene Assay Kit II
RG007S 100 Tests Ask for price

Dual-LumiTM Luciferase Reporter Gene Assay Kit
RG088M 1000 Tests Ask for price

Dual-LumiTM Luciferase Reporter Gene Assay Kit
RG088S 100 Tests Ask for price

Single Firefly Luciferase Reporter Gene Assay Kit
K2236-100 100T
EUR 68
Description: Transcriptional Regulation|Reporter Gene

Single Firefly Luciferase Reporter Gene Assay Kit
K2236-1000 1000T
EUR 336
Description: Transcriptional Regulation|Reporter Gene

Enhanced Firefly Luciferase Reporter Gene Assay Kit
RG009M 1000 Tests Ask for price

Enhanced Firefly Luciferase Reporter Gene Assay Kit
RG009S 100 Tests Ask for price

Renilla-LumiTM Luciferase Reporter Gene Assay Kit
RG062M 1000 Tests Ask for price

Renilla-LumiTM Luciferase Reporter Gene Assay Kit
RG062S 100 Tests Ask for price

Enhanced Firefly Luciferase Reporter Gene Assay Kit II
RG010M 1000 Tests Ask for price

Enhanced Firefly Luciferase Reporter Gene Assay Kit II
RG010S 100 Tests Ask for price

Bio-LumiTM Firefly Luciferase Reporter Gene Assay Kit
RG042M 1000 Tests Ask for price

Bio-LumiTM Firefly Luciferase Reporter Gene Assay Kit
RG042S 100 Tests Ask for price

One-LumiTM Firefly Luciferase Reporter Gene Assay Kit
RG055M 1000 Tests Ask for price

One-LumiTM Firefly Luciferase Reporter Gene Assay Kit
RG055S 100 Tests Ask for price

Renilla-LumiTM Plus Luciferase Reporter Gene Assay Kit
RG066M 1000 Tests Ask for price

Renilla-LumiTM Plus Luciferase Reporter Gene Assay Kit
RG066S 100 Tests Ask for price

Bright-LumiTM Firefly Luciferase Reporter Gene Assay Kit
RG051M 1000 Tests Ask for price

Bright-LumiTM Firefly Luciferase Reporter Gene Assay Kit
RG051S 100 Tests Ask for price

Steady-LumiTM Firefly Luciferase Reporter Gene Assay Kit
RG058M 1000 Tests Ask for price

Steady-LumiTM Firefly Luciferase Reporter Gene Assay Kit
RG058S 100 Tests Ask for price

Gaussia-LumiTM Gaussia Luciferase Reporter Gene Assay Kit
RG072M 1000 Tests Ask for price

Gaussia-LumiTM Gaussia Luciferase Reporter Gene Assay Kit
RG072S 100 Tests Ask for price

Dual-LumiTM II Luciferase Reporter Gene Assay Kit, Dual-LumiTM II Luciferase Assay Kit
RG089M 1000 Tests Ask for price

Dual-LumiTM II Luciferase Reporter Gene Assay Kit, Dual-LumiTM II Luciferase Assay Kit
RG089S 100 Tests Ask for price

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
12518-1plate 1 plate
EUR 166
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
12519-10plates 10 plates
EUR 446
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
12520-100plates 100 plates
EUR 3321
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Bio-LumiTM II Firefly Luciferase Reporter Gene Assay Kit
RG043M 1000 Tests Ask for price

Bio-LumiTM II Firefly Luciferase Reporter Gene Assay Kit
RG043S 100 Tests Ask for price

Bio-LumiTM One Firefly Luciferase Reporter Gene Assay Kit
RG044M 1000 Tests Ask for price

Bio-LumiTM One Firefly Luciferase Reporter Gene Assay Kit
RG044S 100 Tests Ask for price

Bio-LumiTM Steady Firefly Luciferase Reporter Gene Assay Kit
RG046M 1000 Tests Ask for price

Bio-LumiTM Steady Firefly Luciferase Reporter Gene Assay Kit
RG046S 100 Tests Ask for price

Bio-LumiTM One II Firefly Luciferase Reporter Gene Assay Kit
RG045M 1000 Tests Ask for price

Bio-LumiTM One II Firefly Luciferase Reporter Gene Assay Kit
RG045S 100 Tests Ask for price

Dual Luciferase Reporter Gene Assay Cell Lysis Buffer
RG132M 100 mL Ask for price

Dual Luciferase Reporter Gene Assay Cell Lysis Buffer
RG132S 10 mL Ask for price

Bac-LumiTM Bacterial Firefly Luciferase Reporter Gene Assay Kit
RG039M 1000 Tests Ask for price

Bac-LumiTM Bacterial Firefly Luciferase Reporter Gene Assay Kit
RG039S 100 Tests Ask for price

Amplite® Luciferase Reporter Gene Assay Kit *Maximized Luminescence*
12519 10 plates
EUR 455
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Bio-LumiTM Steady II Firefly Luciferase Reporter Gene Assay Kit
RG047M 1000 Tests Ask for price

Bio-LumiTM Steady II Firefly Luciferase Reporter Gene Assay Kit
RG047S 100 Tests Ask for price

Firefly Luciferase Reporter Gene Assay Cell Lysis Buffer
RG126M 100 mL Ask for price

Firefly Luciferase Reporter Gene Assay Cell Lysis Buffer
RG126S 10 mL Ask for price

Renilla Luciferase Reporter Gene Assay Cell Lysis Buffer
RG129M 100 mL Ask for price

Renilla Luciferase Reporter Gene Assay Cell Lysis Buffer
RG129S 10 mL Ask for price

Gaussia Luciferase Reporter Gene Assay Cell Lysis Buffer
RG135M 100 mL Ask for price

Gaussia Luciferase Reporter Gene Assay Cell Lysis Buffer
RG135S 10 mL Ask for price

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12530-1plate 1 plate
EUR 222
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12531-10Plates 10 Plates
EUR 846
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12532-100plates 100 plates
EUR 4447
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12535-1plate 1 plate
EUR 222
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12536-10plates 10 plates
EUR 1070
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12537-100plates 100 plates
EUR 4447
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Luciferase Reporter Assay Kit
GWB-AXR371 200 assays Ask for price

Luciferase Reporter Assay Kit
K2181-200 200 assays
EUR 204.8
Description: Tools Kit|Molecular Biology Kit#Kits|Tools Kit#Kits

Luciferase Reporter Assay Kit
K801-200 each
EUR 235.2

Luciferase Reporter Assay Kit
55R-1540 200 assays Ask for price

One-LumiTM II Firefly Luciferase Reporter Gene Assay Kit, One-LumiTM II Firefly Luciferase Assay Kit
RG056M 1000 Tests Ask for price

One-LumiTM II Firefly Luciferase Reporter Gene Assay Kit, One-LumiTM II Firefly Luciferase Assay Kit
RG056S 100 Tests Ask for price

Bright-LumiTM II Firefly Luciferase Reporter Gene Assay Kit, Bright-LumiTM II Firefly Luciferase Assay Kit
RG052M 1000 Tests Ask for price

Bright-LumiTM II Firefly Luciferase Reporter Gene Assay Kit, Bright-LumiTM II Firefly Luciferase Assay Kit
RG052S 100 Tests Ask for price

Steady-LumiTM II Firefly Luciferase Reporter Gene Assay Kit, Steady-LumiTM II Firefly Luciferase Assay Kit
RG059M 1000 Tests Ask for price

Steady-LumiTM II Firefly Luciferase Reporter Gene Assay Kit, Steady-LumiTM II Firefly Luciferase Assay Kit
RG059S 100 Tests Ask for price

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Maximized Luminescence*
12536 10 plates
EUR 1092
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Enhanced Firefly Luciferase Reporter Gene Assay Cell Lysis Buffer
RG127M 100 mL Ask for price

Enhanced Firefly Luciferase Reporter Gene Assay Cell Lysis Buffer
RG127S 10 mL Ask for price

Dual Luciferase Reporter Assay Kit
DL101-01 100 rxn
EUR 90.5

Dual Luciferase Reporter Assay Kit
DL101-01-100rxns 100 rxns
EUR 93.71

SRE Luciferase Reporter Lentivirus
78627 500 µl x 2
EUR 835
Description: The SRE (Serum Response Element) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the Serum Response Element located upstream of the minimal TATA promoter . After transduction, activation of the MAPK/ERK signaling pathway in the target cells can be monitored by measuring the luciferase activity.

Myc Luciferase Reporter Lentivirus
78628 500 µl x 2
EUR 835
Description: The Myc Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the Myc response element located upstream of the minimal TATA promoter and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the Myc signaling pathway in the target cells can be monitored by measuring the luciferase activity.

p53 Luciferase Reporter Lentivirus
78666 500 µl x 2
EUR 835
Description: The p53 Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by p53 response elements located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, p53-regulated gene expression in the target cells can be monitored by measuring the luciferase activity.

HRE Luciferase Reporter Lentivirus
78668 500 µl x 2
EUR 835
Description: The Hypoxia Response Element (HRE) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by four copies of a hypoxia response elements (HRE) located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the induction of hypoxia in the target cells can be monitored by measuring the luciferase activity.

ARE Luciferase Reporter Lentivirus
79869 500 µl x 2
EUR 875
Description: The Nrf2 antioxidant response pathway plays an important role in the cellular antioxidant defense. Nrf2, a basic leucine zipper transcription factor, induces the expression of antioxidant and phase II enzymes by binding to the ARE (antioxidant response element) region of the gene promoter. Under basal conditions, Nrf2 is retained in the cytosol by binding to the cytoskeletal protein Keap1. Upon exposure to oxidative stress or other ARE activators, Nrf2 is released from Keap1 and translocates to the nucleus, where it can bind to the ARE, leading to the expression of antioxidant and phase II enzymes that protect the cell from oxidative damage.
The ARE Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by ARE located upstream of the minimal TATA promoter. After transduction, activation of the Nrf2 antioxidant response pathway in the target cells can be monitored by measuring the luciferase activity.

TEAD Luciferase Reporter Lentivirus
79833 500 µl x 2
EUR 875
Description: The Hippo pathway regulates cell proliferation and cell death. It is activated by high cell density and cell stress to stop cell proliferation and induce apoptosis. The mammalian Hippo pathway comprises MST kinases and LATS kinases. When the Hippo pathway is activated, MST kinases phosphorylate LATS kinases, which phosphorylate transcriptional co-activators YAP and TAZ. Unphosphorylated YAP and TAZ remain in nucleus and interact with TEAD/TEF transcriptional factors to turn on cell cycle-promoting gene transcription. However, when phosphorylated, YAP and TAZ are recruited from the nucleus to the cytosol, so that the YAP and TAZ-dependent gene transcription is turned off. Dysfunction of the Hippo pathway is frequently detected in human cancer and its down-regulation correlates with the aggressive properties of cancer cells and poor prognosis.
The TEAD Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the TEAD response elements located upstream of the minimal TATA promoter. After transduction, activation of the Hippo pathway in the target cells can be monitored by measuring the luciferase activity._x000D_

STAT3 Luciferase Reporter Lentivirus
79744 500 µl x 2
EUR 860
Description: The STAT3 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of STAT3-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the STAT3 signaling pathway in the target cells can be monitored by measuring the luciferase activity._x000D_

STAT5 Luciferase Reporter Lentivirus
79745 500 µl x 2
EUR 835
Description: The STAT5 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of STAT5-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the STAT5 signaling pathway in the target cells can be monitored by measuring the luciferase activity.

T24 Luciferase Reporter Cell Line
ABC-RC123H each Ask for price

4T1 Luciferase Reporter Cell Line
ABC-RC286H each Ask for price

T84 Luciferase Reporter Cell Line
ABC-RC724H each Ask for price

SCC7 Luciferase Reporter Cell Line
ABC-RC135H each Ask for price

MB49 Luciferase Reporter Cell Line
ABC-RC648H each Ask for price

C1498 Luciferase Reporter Cell Line
ABC-RC160H each Ask for price

TU686 Luciferase Reporter Cell Line
ABC-RC735H each Ask for price

NF-κB Luciferase Reporter Lentivirus
79564 500 µl x 2
EUR 875
Description: The NF-κB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After transduction, activation of the NF-κB signaling pathway in the target cells can be monitored by measuring the luciferase activity.

PC-9 Luciferase Reporter Cell Line
ABC-RC120H each Ask for price

CRE/CREB Luciferase Reporter Lentivirus
79580 500 µl x 2
EUR 835
Description: The main role of the cAMP response element, or CRE, is mediating the effects of Protein Kinase A (PKA) by way of transcription. Upon phosphorylation, CREB forms a functionally active dimer that binds the CRE element within the promoters of target genes and activates transcription. CRE is at the focus of many extracellular and intracellular signaling pathways, including cAMP, calcium, GPCR (G-protein coupled receptors) and neurotrophins. The cAMP/PKA signaling pathway is critical to numerous life processes in living organisms.The CRE/CREB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized cAMP response element (CRE) located upstream of the minimal TATA promoter. After transduction, activation of the cAMP/PKA signaling pathway in the target cells can be monitored by measuring the luciferase activity.

Bald VSV Delta G (Luciferase Reporter)
78636-1 100 µl
EUR 395
Description: The bald VSV Delta G (Luciferase Reporter) was produced without envelope glycoproteins. It contains the firefly luciferase gene as the reporter. The bald VSV Delta G (Luciferase Reporter) can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.

Bald VSV Delta G (Luciferase Reporter)
78636-2 500 µl x 2
EUR 1995
Description: The bald VSV Delta G (Luciferase Reporter) was produced without envelope glycoproteins. It contains the firefly luciferase gene as the reporter. The bald VSV Delta G (Luciferase Reporter) can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.

HuH-7 Luciferase Reporter Cell Line
ABC-RC183H each Ask for price

A-673 Luciferase Reporter Cell Line
ABC-RC568H each Ask for price

C666-1 Luciferase Reporter Cell Line
ABC-RC054H each Ask for price

JIMT-1 Luciferase Reporter Cell Line
ABC-RC628H each Ask for price

NALM-6 Luciferase Reporter Cell Line
ABC-RC665H each Ask for price

B16-OVA Luciferase Reporter Cell Line
ABC-RC578H each Ask for price

MOLM-13 Luciferase Reporter Cell Line
ABC-RC662H each Ask for price

NCI-H520 Luciferase Reporter Cell Line
ABC-RC113H each Ask for price

NIH:OVCAR-3 Luciferase Reporter Cell Line
ABC-RC203H each Ask for price

HCC-1937 Luciferase Reporter Cell Line
ABC-RC305H each Ask for price

NCI-H446 Luciferase Reporter Cell Line
ABC-RC674H each Ask for price

HRE Luciferase Reporter-HeLa Cell Line
RC1018 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The HRE Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the hypoxia response element (HRE). In response to hypoxia (low oxygen), HREs of target genes are recognized and regulated by the hypoxia-inducible factors (HIFs) which belong to the family of basic helix-loop-helix transcription factors and form heterodimeric complex comprising the alpha subunit (HIF-1 alpha, HIF-2 alpha and HIF-3 alpha) and beta subunit (Arnt1, Arnt2 and Arnt3), among which HIF-1 alpha and HIF-2 alpha are predominant isoforms. Activation of HIFs can also be mediated by chemical hydroxylase inhibitors as hypoxia mimetics including the iron chelator desferrioxamine and cobalt chloride.The HRE induction by cobalt chloride is shown in Figure 1.

p53 Luciferase Reporter-HeLa Cell Line
RC1026 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The p53 Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the p53 response element. p53 is a tumor suppressor that plays a crucial role in apoptosis and anticancer mechanisms. p53 reporter system is designed to monitor the p53-mediated signaling pathways.The p53 induction by doxorubicin is shown in Figure 1. 

MRE Luciferase Reporter HEK293 Cell Line
RC1037 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The MRE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the metal response element (MRE). MRE is targeted by MRE-binding transcription factor-1 (MTF-1) which is a zinc finger transcription factor and plays a major role in induction of metallothionein gene expression in response to cellular stress caused by heavy metals such as zinc and cadmium. The MRE induction by ZnSO4 is shown in Figure 1. 

NCI-H1975 Luciferase Reporter Cell Line
ABC-RC338H each Ask for price

Nrf2 Luciferase Reporter-MCF7 Cell Line
RC1017 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The Nrf2 Luciferase Reporter cell line is a stably transfected MCF7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the antioxidant response element (ARE). ARE is known to regulate expression and induction of various detoxifying enzyme genes in response to antioxidants and xenobiotics, and is primarily regulated by the Keap1-Nrf2 pathway in which induction and nuclear translocation of Nrf2 mediated by antioxidants and xenobiotics results in the binding of Nrf2 to ARE leading to the expression of defensive genes. The Nrf2 induction by dimethyl fumarate (DMF) is shown in Figure 1. 

ATF6 Luciferase Reporter-HeLa Cell Line
RC1038 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The ATF6 Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the activating transcription factor 6 (ATF6)-response element. ATF6 is a member of the basic-leucine zipper transcription factor family, which is located in the endoplasmic reticulum (ER) membranes and plays a central role in transcriptional activation of ER molecules.  The ATF6 induction by Tunicamycin is shown in Figure 1. 

EGR1 Promoter Luciferase Reporter Lentivirus
78664 2x 500 µL Ask for price
Description: The Early growth response 1 (EGR1, also known as ZNF268 or NGFi-A) Promoter Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human EGR1 promoter (~1.3 kb, Figure 1) . After transduction, activation of the EGR1 promoter in the target cells can be monitored by measuring the luciferase activity.Figure 1. Schematic of the lenti-vector used to generate the EGR1 Promoter Luciferase Reporter Lentivirus.

U-251 MG Luciferase Reporter Cell Line
ABC-RC354H each Ask for price

STAT1 Luciferase Reporter-HeLa Cell Line
RC1012 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 4536
Description: The STAT1 Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the interferon (IFN) gamma activation sequence-based STAT1 response element, so that the cell line is designed to measure the transcriptional activity of STAT1. As a transcription factor, Signal Transducer and Activator of Transcription 1 (STAT1) is activated through phosphorylation at tyrosine 701 in response to various cytokines and growth factors such as IFN-alpha, IFN-gamma, IL-6, EGF and PDGF. The phosphorylated STAT1 forms homodimers or heterodimers with STAT3, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. The STAT1 induction by IFN-gamma is shown in Figure 1. 

SRE Luciferase Reporter-HEK293 Cell Line
RC1032 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 2772
Description: The SRE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the serum response element (SRE). The SRE reporter cell line is designed to monitor MAPK/ERK activity and can be used for studying GPCR-linked MAPK/ERK signaling pathways as well as screening of agonists, antagonists or signaling inhibitors related with the MAPK/ERK signaling pathways. Functional activity of the cell line has been validated by serum stimulation assay (Figure 1). 

CRE Luciferase Reporter-HEK293 Cell Line
RC1034 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 2772
Description: The CRE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the cAMP response element (CRE). The CRE   cell line is designed to monitor the cAMP/PKA signaling pathways and can be used for studying GPCR-linked cAMP/PKA signaling pathways as well as screening of agonists, antagonists or signaling inhibitors related with the cAMP/PKA signaling pathways. Functional activity of the cell line has been validated by serum stimulation assay (Figure 1). 

SBE Luciferase Reporter-HEK293 Cell Line
RC1036 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The SBE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the smad binding element (SBE). SMADs are intracellular signaling mediators that transduce extracellular signals from transforming growth factor beta (TGF-beta) ligands to the nucleus where they activate downstream gene transcription. The TGF-beta signaling pathway is involved in many cellular processes in both the adult organism and the developing embryo including cell growth, cell differentiation, apoptosis, cellular homeostasis and other cellular functions. The SBE induction by TGF-beta is shown in Figure 1. 

SK-OV-3 Luciferase Reporter Cell Line
ABC-RC149H each Ask for price

NFAT Luciferase Reporter-HEK293 Cell Line
RC1010 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The NFAT Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Nuclear Factor of Activated T-cells (NFAT) response element, so that the cell line is designed to measure the transcriptional activity of NFAT. NFAT is a transcription factor originally found in activated T lymphocytes, and is now known to regulate not only T cell activation and differentiation but also the function of other immune cells including dendritic cells, B cells and megakaryocytes. The NFAT induction by calcium ionophore A23187 is shown in Figure 1. 

NFAT Luciferase Reporter-RAW264.7 Cell Line
RC1011 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The NFAT Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Nuclear Factor of Activated T-cells (NFAT) response element, so that the cell line is designed to measure the transcriptional activity of NFAT. NFAT is a transcription factor originally found in activated T lymphocytes, and is now known to regulate not only T cell activation and differentiation but also the function of other immune cells including dendritic cells, B cells and megakaryocytes. The NFAT induction by calcium ionophore A23187 is shown in Figure 1. 

iNOS Luciferase Reporter-RAW264.7 Cell Line
RC1015 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The iNOS Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the iNOS promoter. Inducible nitric oxide synthase (iNOS) is an inducible enzyme that catalyzes the production of nitric oxide (NO) from L-arginine. NO is one of the smallest signaling molecules that can diffuse into the cell and is involved in various physiological functions, pathogenesis of septic shock, many diseases associated with autoimmunity, and  tumorigenesis. iNOS gene is generally known to be induced by various proinflammatory cytokines and pathogen-associated molecular patterns such as Toll-like receptor (TLR) ligands. The iNOS induction by lipopolysaccharide (LPS), the TLR4 ligand, is shown in Figure 1. 

ISRE Luciferase Reporter-RAW264.7 Cell Line
RC1041 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The ISRE Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Interferon-Stimulated Response Element (ISRE), so that the cell line is designed to monitor the JAK/STAT signaling pathway activity. This cell line can be activated by type I IFNs as well as certain Toll like receptor ligands capable of induction of IRFs such as TLR3 ligand-poly (I:C). Functional activity of the cell line has been validated by poly (I:C) (Figure 1). 

ISRE Luciferase Reporter-HEK293 Cell Line
RC1043 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The ISRE Luciferase Reporter cell line is a stably transfected HEK293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Interferon-Stimulated Response Element (ISRE), so that the cell line is designed to monitor the JAK/STAT signaling pathway activity. Functional activity of the cell line has been validated by IFN-alpha (Figure 1). 

NFAT Luciferase-RFP Reporter Lentivirus
78617-H 500 µl x 2
EUR 835
Description: The NFAT Luciferase-RFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and RFP (Red Fluorescent Protein) cassette driven by the NFAT response element located upstream of the minimal TATA promoter and a hygromycin or puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or RFP expression. RFP fluoresces red-orange when excited; it has an excitation wavelength of 553 nm, and an emission wavelength of 574 nm.

NFAT Luciferase-RFP Reporter Lentivirus
78617-P 500 µl x 2
EUR 835
Description: The NFAT Luciferase-RFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and RFP (Red Fluorescent Protein) cassette driven by the NFAT response element located upstream of the minimal TATA promoter and a hygromycin or puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or RFP expression. RFP fluoresces red-orange when excited; it has an excitation wavelength of 553 nm, and an emission wavelength of 574 nm.

GATA3 Luciferase Reporter-HEK293 Cell Line
RC1009 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The GATA3 Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the GATA3 response element, so that the cell line is designed to measure the transcriptional activity of GATA3. As a zinc-finger transcription factor, GATA3 (GATA-binding protein 3) plays a critical role in early and late T cell differentiation, which regulates Th1/Th2 differentiation. GATA3 has been shown to induce Th2 differentiation and repress Th1 differentiation. GATA3 is also known to promote the secretion of IL-4, IL-5 and IL-13 from Th2 cells. The GATA3 induction by phorbol 12-myristate 13-acetate (PMA)  is shown in Figure 1. 

STAT1 Luciferase Reporter-RAW264.7 Cell Line
RC1013 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 4536
Description: The STAT1 Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the interferon (IFN) gamma activation sequence-based STAT1 response element, so that the cell line is designed to measure the transcriptional activity of STAT1. As a transcription factor, Signal Transducer and Activator of Transcription 1 (STAT1) is activated through phosphorylation at tyrosine 701 in response to various cytokines and growth factors such as IFN-alpha, IFN-gamma, IL-6, EGF and PDGF. The phosphorylated STAT1 forms homodimers or heterodimers with STAT3, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. The STAT1 induction by IFN-gamma is shown in Figure 1. 

STAT3 Luciferase Reporter-HEK293 Cell Line
RC1014 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 4536
Description: The STAT3 Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter under the transcriptional control of the STAT3 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT3. As a transcription factor, Signal Transducer and Activator of Transcription 3 (STAT3) is activated through phosphorylation at tyrosine 705 in response to various cytokines including IL-6, interferons, epidermal growth factor, hepatocyte growth factor and leukemia inhibitory factor. The phosphorylated STAT3 forms homodimers or heterodimers with STAT1, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. The STAT3 induction by IL-6 is shown in Figure 1. 

STAT4 Luciferase Reporter-HEK293 Cell Line
RC1040 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 4536
Description: The STAT4 Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a member of the STAT transcription factor family and plays a central role in generating inflammation during protective immune responses and immune-mediated diseases.  The STAT4 induction by interferon gamma is shown in Figure 1.  

FOXP3 Luciferase Reporter-Jurkat Cell Line
RC1044 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
EUR 1864.8
Description: The FOXP3 Luciferase Reporter cell line is a stably transfected Jurkat T cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Forkhead box P3 (FOXP3) promoter. As a member of the forkhead transcription factor family, FOXP3 is a key transcription factor that functions in the development and function of regulatory T cells. Functional activity of the cell line has been validated by phorbol 12-myristate 13-acetate (PMA) in the presence of ionomycin (Figure 1). 

IL-2 Luciferase Reporter Jurkat cell line
60481 2 vials
EUR 6875
Description: Human IL-2 reporter construct is stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by a human IL-2 promoter.

STAT3 Luciferase Reporter THP-1 Cell Line
78498 2 vials
EUR 1900
Description: The STAT3 Luciferase Reporter THP-1 cell line is designed for monitoring the STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

Transgenic crops have been additionally remodeled with genes from numerous species for drought tolerance. The UK (principally with transgenesis and site-specific nucleases) and France (with no transgenic instruments however with MAS and site-specific nucleases) are the primary nations finishing up analysis applications for each biotic stress and drought tolerance. Thus, few European nations used transgenesis for gluten protein composition and RNAi-mediated silencing in celiac illness. Because of vandalism discipline trials of transgenics dropped since 2000. No transgenic wheat is cultivated in Europe for political causes.