Blueprints for green biotech: development and application of standards for plant synthetic biology.

Blueprints for green biotech: development and application of standards for plant synthetic biology.

Synthetic biology goals to use engineering ideas to the design and modification of organic programs and to the development of organic components and gadgets. The capacity to programme cells by offering new directions written in DNA is a foundational know-how of the sphere. Large-scale de novo DNA synthesis has accelerated synthetic biology by providing custom-made molecules at ever lowering prices. However, for massive fragments and for experiments during which libraries of DNA sequences are assembled in several mixtures, meeting within the laboratory continues to be fascinating.

Biological meeting standards permit DNA components, even these from a number of laboratories and experiments, to be assembled collectively utilizing the identical reagents and protocols. The adoption of such standards for plant synthetic biology has been cohesive for the plant science neighborhood, facilitating the application of genome enhancing applied sciences to plant programs and streamlining progress in large-scale, multi-laboratory bioengineering initiatives. This perspective will display the shift that biotech/pharma is already making and will suggest that the trade has a chance to compel change by adopting some points of the tech trade on the subject of fashions for know-how/product development and management attributes.

Subjects had been divided into 4 teams: Group 1 included 140 diabetic and non-diabetic topics with regular urea and haemoglobin ranges; Group 2 included 84 diabetic and non-diabetic topics with excessive urea ranges; Group three included 44 diabetic and non-diabetic topics with iron deficiency anaemia; and Group four included 52 diabetic and non-diabetic topics with excessive haemoglobin ranges. EP Evaluator Release eight program was used to guage the resultant information. The biotech and pharma (biotech/pharma) trade is in dire want of discovering methods to extend efficacy, effectivity, and affordability of its merchandise. The info and know-how trade (“tech”) trade, which is an trade equally based on innovations and innovation, might present some meals for thought.

 Blueprints for green biotech: development and application of standards for plant synthetic biology.

RABiT-II: Implementation of a High-Throughput Micronucleus Biodosimetry Assay on Commercial Biotech Robotic Systems.

We display the use of high-throughput biodosimetry platforms primarily based on industrial high-throughput/high-content screening robotic programs. The cytokinesis-block micronucleus (CBMN) assay, utilizing solely 20 μl complete blood from a fingerstick, was applied on a PerkinElmer cell::explorer and General Electric IN Cell Analyzer 2000. On common 500 binucleated cells per pattern had been detected by our FluorQuantMN software program. A calibration curve was generated within the radiation dose vary as much as 5.0 Gy utilizing the information from eight donors and 48,083 binucleated cells in complete.
The research described right here demonstrates that high-throughput radiation biodosimetry is sensible utilizing present industrial high-throughput/high-content screening robotic programs, which may be readily programmed to carry out and analyze robotics-optimized cytogenetic assays. Application to different industrial high-throughput/high-content screening programs past those used on this research is clearly sensible. This method will permit a lot wider entry to high-throughput biodosimetric screening for large-scale radiological incidents than is at present accessible.
Keep up with at the moment’s single-use know-how customary but in addition stay one step forward” – that would have been the motto of the BioTech 2016 convention at ZHAW Waedenswil on September 5 and 6, which introduced collectively producers, suppliers, customers, regulatory specialists and representatives of organizations on this discipline to implement single-use gadgets everywhere in the world. It was an effectiveness evaluation of the pattern to exchange reusable gear in manufacturing amenities with single-use elements.
Most of the genetically modified (GM) crops at present commercialized embody a handful of crop species (soybean, corn, cotton and canola) with agronomic characters (traits) directed in opposition to some biotic stresses (pest resistance, herbicide tolerance or each) and created by multinational corporations. The identical crops with agronomic traits already available on the market at the moment will proceed to be commercialized, however there can be additionally a wider vary of species with mixed traits.
The timeframe anticipated for market launch of the following biotech crops is not going to solely depend upon science progress in analysis and development (R&D) in laboratories and fields, but in addition totally on how demanding regulatory necessities are in international locations the place advertising and marketing approvals are pending. Regulatory constraints, together with environmental and well being impression assessments, have elevated considerably previously a long time, delaying approvals and rising their prices. This has generally discouraged public analysis entities and small and medium measurement plant breeding corporations from utilizing biotechnology and given desire to different applied sciences, not as stringently regulated.
Nevertheless, R&D applications are flourishing in growing international locations, boosted by the need to satisfy the worldwide challenges which are meals safety of a booming world inhabitants whereas mitigating local weather change impacts. Biotechnology is an instrument on the service of these imperatives and all kinds of crops are at present examined for their excessive yield regardless of biotic and abiotic stresses. Many crops with greater water or nitrogen use effectivity, tolerant to chilly, salinity or water submergence are being developed. Food safety is just not solely a query of amount but in addition of high quality of agricultural and meals merchandise, to be accessible and accessible for those who want it probably the most.
Many biotech crops (particularly staple meals) are subsequently being developed with dietary traits, akin to biofortification in nutritional vitamins and metals. The principal worldwide seed corporations proceed to be the most important buyers in plant biotechnology R&D, and usually collaborate within the growing world with public establishments, personal entities and philanthropic organizations. These partnerships are significantly current in Africa. In developed international locations, plant biotechnology can also be used for non-food functions, such because the pharmaceutical, biofuel, starch, paper and textile industries.

MAT Activity Assay Kit

abx098901-96tests 96 tests
EUR 1193

GAPDH Activity Assay Kit

K680-100
EUR 256

Urease Activity Assay Kit

55R-2261 100 assays
EUR 689
Description: Assay kit to measure Urease activity

Mitochondria Activity Assay Kit

KC310100 1 kit
EUR 398
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

MAT Activity Assay

IK00401 96 tests
EUR 844
Description: Methionine Adenosyltransferase (MAT) activity assay kit

Caspase-1 Activity Assay Kit

AR4003-100Assays 100 Assays
EUR 514

Caspase-1 Activity Assay Kit

AR4003-25Assays 25 Assays
EUR 236

Caspase-1 Activity Assay Kit

AR4003-50Assays 50 Assays
EUR 340

Caspase-2 Activity Assay Kit

AR4004-100Assays 100 Assays
EUR 514

Caspase-2 Activity Assay Kit

AR4004-25Assays 25 Assays
EUR 236

Caspase-2 Activity Assay Kit

AR4004-50Assays 50 Assays
EUR 340

DAG Kinase Activity Assay Kit

MET-5036 50 assays
EUR 450

Hexokinase (HK) Activity Assay Kit

MET-5087 100 assays
EUR 508

Beta Hexosaminidase Activity Assay Kit

MET-5095 100 assays
EUR 508

OxiSelect ORAC Activity Assay Kit

STA-345 192 assays
EUR 577
Description: The ORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect ORAC Activity Assay measures this capacity in less than 2 hours from a wide variety of sample types.

OxiSelect ORAC Activity Assay Kit

STA-345-5 5 x 192 assays
EUR 2277
Description: The ORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect ORAC Activity Assay measures this capacity in less than 2 hours from a wide variety of sample types.

OxiSelect HORAC Activity Assay Kit

STA-346 192 assays
EUR 577
Description: The HORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect HORAC Activity Assay measures the degradation of free hydroxyl radicals in less than 2 hours from a wide variety of sample types.

OxiSelect HORAC Activity Assay Kit

STA-346-5 5 x 192 assays
EUR 2277
Description: The HORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect HORAC Activity Assay measures the degradation of free hydroxyl radicals in less than 2 hours from a wide variety of sample types.

Calpain Activity Fluorometric Assay Kit

K2062-100 100 assays
EUR 627

KinaseSTAR JNK Activity Assay Kit

K2079-40 40 assays
EUR 738

KinaseSTAR Akt Activity Assay Kit

K2080-40 40 assays
EUR 738

PLTP Activity Fluorometric Assay Kit

K2087-100 100 assays
EUR 669

CETP Activity Fluorometric Assay Kit

K2089-100 100 assays
EUR 725

Proteasome Activity Fluorometric Assay Kit

K2096-100 100 assays
EUR 502

GST Fluorometric Activity Assay Kit

K2105-100 100 assays
EUR 502

GST Colorimetric Activity Assay Kit

K2108-100 100 assays
EUR 529

Trypsin Activity Colorimetric Assay Kit

K2176-100 100 assays
EUR 447

DPP4 Activity Fluorometric Assay Kit

K2178-100 100 assays
EUR 557

Amylase Activity Colorimetric Assay Kit

K2225-100 100 assays
EUR 390

Aconitase Activity Colorimetric Assay Kit

K2226-100 100 assays
EUR 516

Lipase Activity Colorimetric Assay Kit

K2227-100 100 assays
EUR 516

Neuraminidase Activity Fluorometric Assay Kit

K2230-100 100 assays
EUR 599

ACE1 Colorimetric Activity Assay Kit

K2001-100
EUR 555

Glucosylceramidase Activity Assay Kit (Fluorometric)

K2003-100
EUR 588

Transketolase Activity Assay Kit (Fluorometric)

K2004-100
EUR 620

Streptokinase Activity Assay Kit (Colorimetric)

K2008-100
EUR 512

Carboxylesterase Activity Assay Kit (Fluorometric)

K2014-100
EUR 620

Laccase Activity Assay Kit (Colorimetric)

K2038-100 100 assays
EUR 500

CD38 Activity Assay Kit (Fluorometric)

K2042-100 100 assays
EUR 581

Sialyltransferase Activity Assay Kit (Fluorometric)

K2048-100 100 assays
EUR 601

Lysozyme Activity Assay Kit (Fluorometric)

K236-100
EUR 490

Calpain Activity Fluorometric Assay Kit

K240-100
EUR 588

Proteasome Activity Fluorometric Assay Kit

K245-100
EUR 479

GST Fluorometric Activity Assay Kit

K260-100
EUR 468

GST Colorimetric Activity Assay Kit

K263-100
EUR 490

Sirtuin Activity Assay Kit (Fluorometric)

K324-100
EUR 572

HDAC Activity Fluorometric Assay Kit

K330-100
EUR 490

HDAC Activity Colorimetric Assay Kit

K331-100
EUR 490

HAT Activity Colorimetric Assay Kit

K332-100
EUR 539

HAT Activity Fluorometric Assay Kit

K334-100
EUR 539

HDAC3 Activity Fluorometric Assay Kit

K343-100
EUR 479

HDAC8 Activity Fluorometric Assay Kit

K348-100
EUR 490

Chymotrypsin Activity Assay Kit (Fluorometric)

K352-100
EUR 490

Thrombin Activity Fluorometric Assay Kit

K373-100
EUR 697

Urease Activity Assay Kit (Colorimetric)

K378-100
EUR 501

Plasmin Activity Assay Kit (Fluorometric)

K381-100
EUR 457

ATPase Activity Assay Kit (Colorimetric)

K417-100
EUR 430

JNK Activity Assay Kit, KinaseSTAR

K431-40
EUR 664

Akt Activity Assay Kit, KinaseSTAR

K435-40
EUR 680

Lysosomal Intracellular Activity Assay Kit

K448-50
EUR 544

HDAC6 Activity Assay Kit (Fluorometric)

K466-100
EUR 631

Deubiquitinase Activity Assay Kit (Fluorometric)

K485-100
EUR 533

Neprilysin Activity Assay Kit (Fluorometric)

K487-100
EUR 610

Chitotriosidase Activity Assay Kit (Fluorometric)

K512-100
EUR 620

?-Glucuronidase Activity Assay Kit (Fluorometric)

K514-100
EUR 620

Methyltransferase Activity Assay Kit (Fluorometric)

K521-100
EUR 566

Transglutaminase Activity Assay Kit (Colorimetric)

K571-100
EUR 718

Sphingomyelinase Activity Fluorometric Assay Kit

K574-100
EUR 457

Fumarase Activity Colorimetric Assay Kit

K596-100
EUR 653

Sphingomyelinase Activity Colorimetric Assay Kit

K599-100
EUR 446

Aldolase Activity Colorimetric Assay Kit

K665-100
EUR 664

Ceruloplasmin Activity Colorimetric Assay Kit

K669-100
EUR 550

Invertase Activity Colorimetric Assay Kit

K674-100
EUR 490

Sulfatase Activity Assay Kit (Colorimetric)

K675-100
EUR 653

?-Glucosidase Activity Colorimetric Assay Kit

K690-100
EUR 550

Amylase Activity Colorimetric Assay Kit

K711-100
EUR 408

Aconitase Activity Colorimetric Assay Kit

K716-100
EUR 501

Lipase Activity Colorimetric Assay Kit

K722-100
EUR 512

Urokinase Activity Fluorometric Assay Kit

K728-100
EUR 544

Neuraminidase Activity Fluorometric Assay Kit

K732-100
EUR 523

Uricase Activity Assay Kit (Fluorometric)

K734-100
EUR 512

Tyrosinase Activity Assay Kit (Colorimetric)

K742-100
EUR 582

Arginase Activity Colorimetric Assay Kit

K755-100
EUR 539

Acetylcholinesterase Activity Colorimetric Assay Kit

K764-100
EUR 550

Trypsin Activity Colorimetric Assay Kit

K771-100
EUR 452

DPP4 Activity Fluorometric Assay Kit

K779-100
EUR 544

Protease Activity Fluorometric Assay Kit

K781-100
EUR 387

Collagenase Activity Colorimetric Assay Kit

K792-100
EUR 408

Renin Activity Fluorometric Assay Kit

K800-100
EUR 523

Plasmin Activity Assay Kit (Colorimetric)

K945-100
EUR 446

Cholinesterase Activity Assay Kit (Colorimetric)

K975-100
EUR 642

Lipoxygenase Activity Assay Kit (Fluorometric)

K978-100
EUR 653

?-Xylosidase Activity Assay Kit (Fluorometric)

K981-100
EUR 588

Methyltransferase Activity Assay Kit (Colorimetric)

K986-100
EUR 648

GST Colorimetric Activity Assay Kit

55R-1353 100 assays
EUR 706
Description: Assay Kit for detection of GST activity in the research laboratory

Beta Secretase Activity Assay Kit

55R-1383 100 assays
EUR 835
Description: Assay Kit for detection of Beta Secretase Activity in the research laboratory
For instance, crops are modified to particularly produce molecules with therapeutic makes use of, or with an improved biomass conversion effectivity, or producing bigger volumes of feedstocks for biofuels. Various plant breeding applied sciences are actually utilized in your entire spectrum of plant biotechnology: transgenesis producing proteins or RNAi. Cisgenesis (transgenes remoted from a crossable donor plant) and intragenesis (transgenes originate from the identical species or a crossable species), null segregants are additionally used. To date, the following technology precision gene enhancing instruments are developed in primary analysis. They embody: clustered recurrently interspaced brief palindromic repeats (CRISPR), oligonucleotide-directed mutagenesis (ODM), transcription activator-like results nucleases (TALENs) and zinc-finger nuclease (ZFN).

 

Additive Biotech-Chances, challenges, and recent applications of additive manufacturing technologies in biotechnology.

Additive Biotech-Chances, challenges, and recent applications of additive manufacturing technologies in biotechnology.

The variety and complexity of biotechnological applications are continually rising, with ever increasing ranges of manufacturing hosts, cultivation situations and measurement duties. Consequently, many analytical and cultivation programs for biotechnology and bioprocess engineering, similar to microfluidic units or bioreactors, are tailored to exactly fulfill the necessities of particular measurements or cultivation duties. Additive manufacturing (AM) technologies supply the likelihood of fabricating tailored 3D laboratory tools instantly from CAD designs with beforehand inaccessible ranges of freedom in phrases of structural complexity.

This assessment discusses the historic background of these technologies, their most promising present implementations and the related workflows, fabrication processes and materials specs, along with some of the key challenges related to utilizing AM in biotechnology/bioprocess engineering. To illustrate the good potential of AM, chosen examples in microfluidic units, 3D-bioprinting/biofabrication and bioprocess engineering are highlighted.

Cuban advances in biotech have made headlines, notably for the reason that US-Cuba rapprochement and signing of the historic memorandum of understanding between the US Department of Health and Human Services and Cuba’s Ministry of Public Health in June. Some 34 Cuban establishments with 22,000 workers are the spine of a biotech trade that dates to the early 1980s, acquiring novel merchandise which have sparked curiosity amongst potential international companions. While a quantity of these Cuban merchandise are registered in numerous nations, their testing in the USA stays ensnared in the pink tape of embargo legal guidelines that are inclined to make buyers skittish and thus delay, if not curtail, joint analysis and scientific trial applications to the FDA.

Safety Profile of Anticancer and Immune-Modulating Biotech Drugs Used in a Real World Setting in Campania Region (Italy): BIO-Cam Observational Study.

To examine the prevalence of hostile occasions (AEs) in naïve sufferers receiving biotech medication. Design: A potential observational examine. Setting: Onco-hematology, Hepato-gastroenterology, Rheumatology, Dermatology, and Neurology Units in Campania Region (Italy). Participants: 775 sufferers (53.81% feminine) with imply age 56.0 (SD 15.2). The imply follow-up/affected person was 3.48 (95% confidence interval 3.13-3.84). Main consequence measures: We collected all AEs related to biotech medication, together with severe infections and malignancies.

Serious AEs have been outlined in keeping with the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use, scientific security knowledge administration: definitions and requirements for expedited reporting E2A guideline. Results: The majority of the examine inhabitants was enrolled in Onco-hematology and Rheumatology Units and the commonest analysis have been hematological malignancies, adopted by rheumatoid arthritis, colorectal most cancers, breast most cancers, and psoriatic arthritis.

The mostly prescribed biotech medication have been rituximab, bevacizumab, infliximab, trastuzumab, adalimumab, and cetuximab. Out of 775 sufferers, 320 skilled a minimum of one AE. Most of sufferers skilled AEs to cetuximab remedy, rituximab and trastuzumab. Comparing feminine and male inhabitants, our findings highlighted a statistically important distinction in phrases of AEs for adalimumab (35.90% vs. 7.41%, p < 0.001) and etanercept (27.59% vs. 10.00%, p = 0.023). Considering all biotech medication, we noticed a peak for all AEs prevalence at follow-up 91-180 days class. Bevacizumab, brentuximab, rituximab, trastuzumab and cetuximab have been extra generally related to severe hostile occasions; most of these have been probably associated to biotech medication, in keeping with causality evaluation.

Three circumstances of severe infections occurred. Conclusions: The outcomes of our examine demonstrated that almost all of AEs weren’t severe and anticipated. Few circumstances of severe infections occurred, whereas no case of malignancy did. Overall, the protection profile of biotech medication used in our inhabitants was much like these noticed in pivotal trials. Notwithstanding the optimistic outcomes of our examine, some security issues nonetheless stay unresolved. In order to gather extra effectiveness and security knowledge on biotech medication, the gathering and evaluation of actual world knowledge needs to be endorsed in addition to the administration of post-authorization research.

 Additive Biotech-Chances, challenges, and recent applications of additive manufacturing technologies in biotechnology.

What Will Be the Benefits of Biotech Wheat for European Agriculture?

In European nations, wheat occupies the biggest crop space with excessive yielding manufacturing. France, a significant producer and exporter in Europe, ranks the fifth producer worldwide. Biotic stresses are European farmers’ main challenges (fungal and viral ailments, and insect pests) adopted by abiotic ones similar to drought and grain protein composition. During the final 40 years, 1136 scientific articles on biotech wheat have been printed by USA adopted by China, Australia, Canada, and European Union with the UK. European analysis focuses on pests and ailments resistances utilizing broadly marker-assisted choice (MAS). Transgenesis is used in fundamental analysis to develop resistance towards some fungi (Fusarium head blight) whereas RNA interference (RNAi) silencing is used towards some fungi and virus.

Luciferase Reporter Gene Assay Kit

Z5030003 1,000 assays
EUR 1100
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

SuperLight Luciferase Reporter Gene Assay Kit

SLLU-01K 1000
EUR 502
Description: Bright bioluminescent reagent system allows rapid quantitation of luciferase reporter gene expression in transfected cells within 2 min. Kit size: 1000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

SuperLight Luciferase Reporter Gene Assay Kit

SLLU-200 200
EUR 200
Description: Bright bioluminescent reagent system allows rapid quantitation of luciferase reporter gene expression in transfected cells within 2 min. Kit size: 200 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

SuperLight Luciferase Reporter Gene Assay Kit

SLLU-500 500
EUR 318
Description: Bright bioluminescent reagent system allows rapid quantitation of luciferase reporter gene expression in transfected cells within 2 min. Kit size: 500 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

SuperLight Dual Luciferase Reporter Gene Assay Kit

SLDL-100 100
EUR 200
Description: Bioluminescent reagent system allows rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells within 10 min. Kit size: 100 tests. Shelf life: 12 months. Shipping: ambient temp; storage: -20°C.

Luciferase Reporter Assay Kit

K2181-200 200 assays
EUR 181

Luciferase Reporter Assay Kit

K801-200
EUR 196

Luciferase Reporter Assay Kit

55R-1540 200 assays
EUR 245
Description: Assay Kit for detection of Luciferase Reporter in the research laboratory

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12518 1 plate
EUR 176

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12519 10 plates
EUR 393

Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*

12520 100 plates
EUR 2616

Single-Luciferase Reporter Assay Kit

20-abx098133
  • EUR 566.00
  • EUR 472.00
  • 200 rxns
  • 50 rxns

Double-Luciferase Reporter Assay Kit

20-abx098134
  • EUR 754.00
  • EUR 519.00
  • 200 rxns
  • 50 rxns

Dual Luciferase Reporter Assay Kit

DL101-01 100 rxn
EUR 258

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12530 1 plate
EUR 219

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12531 10 Plates
EUR 702

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12532 100 plates
EUR 3486

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12535 1 plate
EUR 219

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12536 10 plates
EUR 876

Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12537 100 plates
EUR 3486

Single-Luciferase (Renilla) Reporter Assay Kit

20-abx298011
  • EUR 457.00
  • EUR 269.00
  • 200 rxns
  • 50 rxns

TFEB promoter-luciferase reporter

PVT18227 2 ug
EUR 300

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941

AlleleBalanced Luciferase Assay Kit

ABP-PA-ABLA001 100 RXNS Ask for price

AlleleBalanced Luciferase Assay Kit

ABP-PA-ABLA011 1000 RXNS Ask for price

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941

Firefly Luciferase Assay Kit (Lyophilized)

30075-1 150TST
EUR 125
Description: Minimum order quantity: 1 unit of 150TST

Firefly Luciferase Assay Kit (Lyophilized)

30075-2 1000TST
EUR 459
Description: Minimum order quantity: 1 unit of 1000TST

Renilla Luciferase Assay Kit 2.0

30082-1 150AS
EUR 172
Description: Minimum order quantity: 1 unit of 150AS

Renilla Luciferase Assay Kit 2.0

30082-2 1000AS
EUR 594
Description: Minimum order quantity: 1 unit of 1000AS

Renilla Luciferase Assay Kit 2.0

30082-T 50AS
EUR 114
Description: Minimum order quantity: 1 unit of 50AS

Firefly Luciferase Assay Kit 2.0

30085-1 150AS
EUR 125
Description: Minimum order quantity: 1 unit of 150AS

Firefly Luciferase Assay Kit 2.0

30085-2 1000AS
EUR 459
Description: Minimum order quantity: 1 unit of 1000AS

Firefly Luciferase Assay Kit 2.0

30085-T 50AS
EUR 109
Description: Minimum order quantity: 1 unit of 50AS

LucyQ Firefly Luciferase Assay Kit

L0010-010 100 Assays
EUR 273

LucyQ Firefly Luciferase Assay Kit

L0010-100 1000 Assays
EUR 617

LucyQ Gaussia Luciferase Assay Kit

L0011-010 100 Assays
EUR 289

LucyQ Gaussia Luciferase Assay Kit

L0011-100 1000 Assays
EUR 944

LucyQ Renilla Luciferase Assay Kit

L0012-010 100 Assays
EUR 273

LucyQ Renilla Luciferase Assay Kit

L0012-100 1000 Assays
EUR 617

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2132

CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent)

CASCL9-100A-KIT 1 Kit
EUR 1132

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Minicircle Single Reporter: CMV-Luciferase Minicircle (30 ug)

BLIV510MC-1 30 ug
EUR 716

Minicircle Single Reporter: EF1 Luciferase DNA (30 ug)

BLIV511MC-1 30 ug
EUR 716

pMIR- Reporter Plasmid

PVT1324 2 ug
EUR 266

2C::tdTomato Reporter

PVT10473 2 ug
EUR 266

Firefly & Renilla Luciferase Single Tube Assay Kit

30081-1 150AS
EUR 244
Description: Minimum order quantity: 1 unit of 150AS

Firefly & Renilla Luciferase Single Tube Assay Kit

30081-2 1000AS
EUR 984
Description: Minimum order quantity: 1 unit of 1000AS

Firefly & Renilla Luciferase Single Tube Assay Kit

30081-T 50AS
EUR 155
Description: Minimum order quantity: 1 unit of 50AS

LucyQ Duo-Luciferase(Firefly & Renilla) Assay Kit

L0013-010 100 Assays
EUR 317

LucyQ Duo-Luciferase(Firefly & Renilla) Assay Kit

L0013-100 1000 Assays
EUR 1848

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

RNA/DNA Purification Kit (Magnetic Bead)

DA0623 32 test/kit Ask for price

PCR detection kit for Monkeypox Virus

DA1430 24 test/kit
EUR 108
Description: Monkeypox is a viral zoonosis (a virus transmitted to humans from animals) with symptoms very similar to those seen in the past in smallpox patients, although it is clinically less severe. It is caused by the monkeypox virus which belongs to the orthopoxvirus genus of the Poxviridae family. There are two clades of monkeypox virus: the West African clade and the Congo Basin (Central African) clade. The name monkeypox originates from the initial discovery of the virus in monkeys in a Danish laboratory in 1958. The first human case was identified in a child in the Democratic Republic of the Congo in 1970.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Minicircle Single Reporter: CMV-Luciferase Parental Plasmid (10 ug)

BLIV510MN-1 10 ug
EUR 972

BLIV 2.0 Reporter: CMV-Luciferase-EF1a-copGFP Lentivector Plasmid

BLIV511PA-1 10 ug
EUR 803

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110

pMIR-Reporter-RASA1(3

PVTB00444-2a 2 ug
EUR 356

pMIR-Reporter-IL13(3

PVTB00445-2a 2 ug
EUR 356

pGL3- FOXO- Reporter- Luc

PVT10792 2 ug
EUR 301

pTAL- p53- Reporter- Luc

PVT10822 2 ug
EUR 301

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1132

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 805

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1132

Lactose Assay Kit

MET-5001 100 assays
EUR 432
Description: The Lactose Assay Kit measures total lactose in milk based food products or biological samples such as blood or urine. Lactose is cleaved into glucose and galactose. Glucose is then oxidized, yielding hydrogen peroxide and D-gluconic acid. The hydrogen peroxide is detected by a fluorometric probe.

Bilirubin Assay Kit

MET-5010 200 assays
EUR 479
Description: Bilirubin, a byproduct of heme breakdown, can exist conjugated to glucuronic acid (direct) and as unconjugated (indirect). The unconjugated form is found in the blood bound to albumin and is transported to the liver. Bilirubin becomes conjugated to glucuronic acid in the liver, making it more soluble and allowing for excretion into bile. High levels of bilirubin have been correlated with jaundice and Gilbert?s syndrome while low levels have been associated with cardiovascular disease and diabetes mellitus.

Pyruvate Assay Kit

MET-5029 100 assays
EUR 479
Description: Our Pyruvate Assay Kit measures pyruvate in biological samples. First, pyruvate is oxidized by pyruvate oxidase, producing hydrogen peroxide. The hydrogen peroxide is then detected at ex. 530-570 nm/em. 590-600 nm using a specific fluorometric probe. Pyruvate levels in unknown samples are determined based on the provided pyruvate standard curve.

Glycine Assay Kit

MET-5070 100 assays
EUR 450

Taurine Assay Kit

MET-5071 200 assays
EUR 508

Sarcosine Assay Kit

MET-5072 100 assays
EUR 450

Tyrosine Assay Kit

MET-5073 100 assays
EUR 450

Phospholipid Assay Kit

MET-5085 96 assays
EUR 456

Ammonia Assay Kit

MET-5086 100 assays
EUR 456

Adenosine Assay Kit

MET-5090 100 assays
EUR 508

Inosine Assay Kit

MET-5092 100 assays
EUR 508

Alanine Assay Kit

MET-5093 200 assays
EUR 508

Histamine Assay Kit

AKR-360 96 assays
EUR 519
Description: Histamine is naturally occurring in food, with high concentrations associated with spoiled and fermented foods. Exposure to high levels of histamine through the ingestion of food can cause symptoms similar to an allergic response. Our Histamine Assay Kit detects total histamine from food samples using a colorimetric probe. Reduction of the probe yields color development proportional the histamine levels in the sample. Absorbance at 450nm is read after a one hour incubation at 37C and histamine levels are calculated based on a histamine standard curve.

Glucose Assay Kit

abx090673-1Kit 1 Kit
EUR 237

ADA Assay Kit

abx090675-100tests 100 tests
EUR 237

Glutamate Assay Kit

abx096004-100Assays 100 Assays
EUR 441

Glutathione Assay Kit

abx096005-100Assays 100 Assays
EUR 378

Trehalase Assay Kit

abx096014-100Assays 100 Assays
EUR 551

Pyruvate Assay Kit

abx097982-100Assays 100 Assays
EUR 472

NADPase Assay Kit

abx097983-100Assays 100 Assays
EUR 504

Starch Assay Kit

abx097988-100Assays 100 Assays
EUR 441

Trehalose Assay Kit

abx097995-100Assays 100 Assays
EUR 472

ADA Assay Kit

abx098403-Hitachi7060R190ml2R290ml1 Hitachi 7060; R1: 90ml×2 R2: 90ml×1
EUR 739

Transgenic crops have been additionally remodeled with genes from numerous species for drought tolerance. The UK (principally with transgenesis and site-specific nucleases) and France (with no transgenic instruments however with MAS and site-specific nucleases) are the primary nations finishing up analysis applications for each biotic stress and drought tolerance. Thus, few European nations used transgenesis for gluten protein composition and RNAi-mediated silencing in celiac illness. Because of vandalism discipline trials of transgenics dropped since 2000. No transgenic wheat is cultivated in Europe for political causes.

Chemistry, sustainability and naturality of perfumery biotech ingredients

Chemistry, sustainability and naturality of perfumery biotech ingredients

White biotechnology has emerged in biochemical manufacturing processes to ship perfumery ingredients satisfying novel pursuits of the society for pure, eco-responsible and sustainable supplies. As a consequence, an intense R&D exercise has taken place on these topics, leading to each scientific publications and patent functions reporting combos of state-of-the-art approaches in biocatalysis, metabolic engineering, artificial biology, biosynthesis elucidation, gene version and cloning, and analytical chemistry.

In this minireview, we current a smelly choice of novel biotechnological processes and ingredients from a scientific articles and patents survey protecting the final 6 years and analysed in phrases of chemistry, sustainability and naturalitysynthesis of important oil surrogates or single molecule ingredients, and on one other aspect the optimisation of properties of pure advanced substances by particular and selective enzymatic modifications of their chemical composition. Data had been confirmed, even evaluating luggage based on filling quantity. Data introduced on this research help the conclusion that CryoMACS freezing luggage are bioequivalent to Maco Biotech Freezing-EVA Bags for HPC cryopreservation.

The growing discoveries concerning the biology and capabilities of platelets within the final decade undoubtedly present that these cells are one of probably the most biotechnological human cells. This evaluate summarizes new advances in platelet biology, capabilities, and new ideas of biotech-educated platelets that join superior biomimetic science to platelet-based additive manufacturing for tissue regeneration. As extremely responsive and secretory cells, platelets may very well be explored to develop options that alter injured microenvironments by platelet-based artificial biomaterials with instructive extracellular cues for morphogenesis in tissue engineering past tissue regeneration 2.0.

Comparison of CryoMACS Freezing Bags with Maco Biotech Freezing-Ethinyl Vinyl Acetate Bags for Hematopoietic Progenitor Cells Cryopreservation Using a CD34 +-Enriched Product

Hematopoietic progenitor cells (HPCs) cryopreservation have functions, particularly within the autologous setting, permitting therapeutic use a number of years after assortment. Cryopreservation goals to protect the therapeutic properties of HPCs, and profitable cryopreservation will depend on a number of elements akin to preservation procedures, biopreservation media, freezing charges, and thawing procedures. In this context, the selection of the freezing bag is important because it offers mechanical safety through the freezing course of. Since Maco Biotech Freezing-ethinyl vinyl acetate (EVA) Bags® are now not obtainable in our nation, a comparative research was developed to confirm bioequivalence with the Miltenyi CryoMACS® freezing bag.

In this research, a CD34+-enriched product was used to higher reproduce HPC apheresis. Freezing luggage had been crammed with the identical quantity, cryopreserved with managed price freezing, and saved within the vapor part of liquid nitrogen for no less than 6 months. After thawing, all luggage had been examined for . Classification has been made between metabolic engineering on one aspect, permitting both biotechnological  integrity and sterility utilizing a microbial problem. In addition, a comparability was developed by evaluating restoration of white blood cells, mononuclear cells, lymphocytes, and CD34+ cells.No important variations between the 2 producers’ luggage have been noticed in phrases of the evaluated parameters.

This mini-review offers a perspective of conventional, rising and future functions of lactic acid micro organism (LAB) and how genome modifying instruments can be utilized to beat present challenges in all these functions. It additionally describes obtainable instruments and how these might be additional developed, and takes present laws under consideration. Genome modifying instruments are obligatory for the development of strains for brand spanking new functions and merchandise, however may play an important function in conventional ones, akin to meals and probiotics, as a analysis software for gaining mechanistic insights and discovering new properties.

Traditionally, recombinant DNA methods for LAB have strongly centered on being food-grade, however they lack velocity and the quantity of genetically tractable strains continues to be reasonably restricted. Further software growth will allow fast building of a number of mutants or mutant libraries on a genomic degree in all kinds of LAB strains. We additionally suggest an iterative Design-Build-Test-Learn workflow cycle for LAB cell manufacturing facility growth based mostly on techniques biology, with ‘cell manufacturing facility’ increasing past its conventional which means of manufacturing strains and making use of genome modifying instruments to advance LAB understanding, functions and pressure growth.

 Chemistry, sustainability and naturality of perfumery biotech ingredients

Microbe Profile: Komagataella phaffii: a methanol devouring biotech yeast previously generally known as Pichia pastoris

Methylotrophic yeasts of the genus Komagataella are abundantly present in tree exudates. Their potential to make the most of methanol as carbon and power supply depends on an assimilation pathway localized in largely expanded peroxisomes, and a cytosolic methanol dissimilation pathway. Other substrates like glucose or glycerol are readily utilized as effectively. Upon mating diploid cells sporulate readily, forming asci with 4 haploid spores. Their potential to secrete excessive quantities of heterologous proteins made them attention-grabbing for biotechnology, which expands as we speak additionally to different merchandise of major and secondary metabolism.

96 well filter plate (960ul each well)

SD5006 12UNIT
EUR 96.54

96 WELL CLEAR PCR PLATE FOR ABI

PCR-96-AB-C 10/pk
EUR 167
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY

P-DW-12-HC 5/pk
EUR 152
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

96 well filter plate (no filter)

SD5006-EMPTY 12UNIT
EUR 87.41

Clear Coated 96 Well Plate, 1EA

C107-1EA 1EA
EUR 189

96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE

PCR-96-HS-C 10/pk
EUR 159
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE.

CP-AXYGEM-96-50 10/pk
EUR 457
Description: Crystallography Plates; Crystallography Plates - Axygen

Equine Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-Eq-48T 48T
EUR 360
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-Eq-96T 96T
EUR 457
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-Eq-48Tests 48 Tests
EUR 358

Equine Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-Eq-96Tests 96 Tests
EUR 490

96 well DNA plate with membrane (960ul each well)

SD5007 12UNIT
EUR 242.7

96 well RNA plate with membrane (960ul each well)

SD5009 12UNIT
EUR 260.97

96-Well Plate PCR Products Purification Kit

BS365 5XPlates, 480prep
EUR 250.31

96-Well Plate PCR Products Purification Kit

BS3652 2xPlates, 192prep
EUR 155.16

96-Well Plate DNA Cleanup Miniprep Kit

BS369 5XPlates, 480prep
EUR 250.31

96-Well Plate DNA Cleanup Miniprep Kit

BS3692 2xPlates, 192prep
EUR 155.16

96-Well Plate Plasmid DNA Miniprep Kit

BS415 5XPlates, 480prep
EUR 250.31

96-Well Plate Plasmid DNA Miniprep Kit

BS4152 2XPlates, 192prep
EUR 155.16

96-Well Plate Viral DNA Miniprep Kit

VT92032 2XPlates, 192prep
EUR 413.22

Polystyrene-bottomed 96-well plate + lid (ELISPOT)

CT365 5
EUR 78

2 x Clear 96 well Plate, 2EA

X003-2EA 2EA
EUR 65

5 x Clear 96 well Plate, 5EA

X003-5EA 5EA
EUR 80

2 x Clear 96 well Plate, 2EA

X018-2EA 2EA
EUR 65

4 x Clear 96 Well Plate, 4EA

X018-4EA 4EA
EUR 87

Black Half Area 96 Well Plate, 1EA

X023-1EA 1EA
EUR 80

96 Well Breast Tumor Genomic DNA Plate

D8235086-1 1 plate
EUR 316

96 Well Colon Tumor Genomic DNA Plate

D8235090-1 1 plate
EUR 316

96 Well Lung Tumor Genomic DNA Plate

D8235152-1 1 plate
EUR 316

96 Well Stomach Tumor Genomic DNA Plate

D8235248-1 1 plate
EUR 316

Radius 96-Well Cell Migration Assay

CBA-126 96 assays
EUR 572
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126-5 5 x 96 assays
EUR 2248
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 96-well Cell Transformation Assay

CBA-130 96 assays
EUR 722
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay

CBA-130-5 5 x 96 assays
EUR 2886
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Phagocytosis Assay (Zymosan)

CBA-224 96 assays
EUR 722
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-well Phagocytosis Assay (Zymosan)

CBA-224-5 5 x 96 assays
EUR 2938
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

OxiSelect 96-Well Comet Assay Kit

STA-355 96 assays
EUR 427
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Kit

STA-355-5 5 x 96 assays
EUR 1633
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Slide

STA-356 1 slide
EUR 183
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect 96-Well Comet Assay Slide

STA-356-5 5 slides
EUR 519
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

96-Well ROCK Activity Assay Kit

STA-416 96 assays
EUR 856
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96-Well ROCK Activity Assay Kit

STA-416-5 5 x 96 assays
EUR 3524
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED

P-DW-12-HC-S 5/pk
EUR 207
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included)

ACT-SL-96
EUR 647

96-well Plasmid DNA Extraction Kit (1 plate)

FAPWE-96001 1 prep
EUR 146

96 Well Plate RNA Cleanup and Concentration Kit

BRC1251 2xPlates, 192prep
EUR 250.31

96-Well Plate Animal Genomic DNA Miniprep Kit

BS437 5XPlates, 480prep
EUR 362.98

96-Well Plate Animal Genomic DNA Miniprep Kit

BS4372 2XPlates, 192prep
EUR 195.5

96 Well Plate Yeast Genomic DNA Miniprep Kit

BS8357 2XPlates, 192prep
EUR 195.5

96-Well Plate Plant Genomic DNA Miniprep Kit

BS8361 2XPlates, 192prep
EUR 195.5

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92031 2Plates, 192prep
EUR 175.71

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92032 5Plates, 480prep
EUR 324.92

Block for 96 well plate, WSC-2620/30

4002659 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1292 2XPlates, 192prep
EUR 175.71

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1295 5XPlates, 480prep
EUR 324.92

PVDF membrane-bottomed 96-well plate + lid (ELISPOT)

CT366 5
EUR 224

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)

EXOEL-96P-1 10 Plates
EUR 213

CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric)

CBA-081 96 assays
EUR 606
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.

96-well Checkpoint Kinase Activity Assay Kit

STA-414 96 assays
EUR 757
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-well Checkpoint Kinase Activity Assay Kit

STA-414-5 5 x 96 assays
EUR 3060
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-Well Assay Plates, Non-Treated, Clear, Round Bottom 1 Plate/Pack, 100/Unit

25-224 100 plates
EUR 299
Description:

Suitable for cell culture but only for suspension cells

  • Optically clear with low autofluorescence for distortion free microscopy
  • Low evaporation lid reduces evaporation and prevents contamination
  • Exterior stacking ring on lid for added stability when stacking
  • Certified RNase & DNase-Free, Non-Pyrogenic & Non-Cytotoxic
  • Sterilized by Gamma Irradiation to a Sterility Assurance Level of 10^-10

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTA55-5P 1 PK
EUR 263

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTG65-5P 1 PK
EUR 263

Adaptor for 96-well PCR plate, accessory for AgileSealer?

APS-200-96PCR
EUR 351

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS585 5XPlates, 480prep
EUR 487.82

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS5852 2XPlates, 192prep
EUR 250.31

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92028 2XPlates, 192prep
EUR 212.25

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92029 5XPlates, 480prep
EUR 364.5

Block for 96 deep-well plate, WSC-2620/30

4002661 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE

432071 1/pk
EUR 345
Description: BioCision; BioCision CoolBox

SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED

480130 1/pk
EUR 223
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES

480131 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED

480132 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

96 Well PCR Plates, Fits ABI Fast Plate Machines

GP615 25 plates
EUR 212

Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA

C075-1EA 1EA
EUR 178

Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA

C140-1EA 1EA
EUR 178

Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA

C223-1EA 1EA
EUR 178

Goat anti-Mouse, Coated White 96 Well Plate, 1EA

X010-1EA 1EA
EUR 109

Goat anti-Rabbit, Coated White 96 Well Plate, 1EA

X014-1EA 1EA
EUR 109

Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA

X016-1EA 1EA
EUR 109

2 x Black Half Area 96 Well Plate, 2EA

X037-2EA 2EA
EUR 94

Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA

X061-1EA 1EA
EUR 109

Donkey anti-Sheep, Coated White 96 Well Plate, 1EA

X063-1EA 1EA
EUR 109

CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (96 assays)

CBA-320 96 assays
EUR 537
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML

PCR-96-AB-C-PBC 10/pk
EUR 195
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

DH10B Chemically Competent Cells (ig10B) - 1x96 well plate - 20µl/well

1018-96 1/EA
EUR 518

Clear 384 Well Plate

X105-1EA 1EA
EUR 112

CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric

CBA-112-COL 96 assays
EUR 757
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric

CBA-112-LN 96 assays
EUR 757
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Phagocytosis Assay (Red Blood Cell)

CBA-220 96 assays
EUR 635
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

96-well Cellular Senescence Assay (SA ?-Gal Activity)

CBA-231 120 assays
EUR 635
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

96-well Cellular Senescence Assay (SA ?-Gal Activity)

CBA-231-5 5 x 120 assays
EUR 2648
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

Targeted genome modifying is a sophisticated method that allows exact modification of the nucleic acid sequences in a genome. Genome modifying is usually carried out utilizing instruments, akin to molecular scissors, to chop an outlined location in a selected gene. Genome modifying has impacted numerous fields of biotechnology, akin to agriculture; biopharmaceutical manufacturing; research on the construction, regulation, and perform of the genome; and the creation of transgenic organisms and cell strains. Although genome modifying is used often, it has a number of limitations.

Here, we offer an outline of well-studied genome-editing nucleases, together with single-stranded oligodeoxynucleotides (ssODNs), transcription activator-like effector nucleases (TALENs), zinc-finger nucleases (ZFNs), and CRISPR-Cas9 RNA-guided nucleases (CRISPR-Cas9). To this finish, we describe the progress towards editable nuclease-based therapies and talk about the minimization of off-target mutagenesis. Future prospects of this difficult scientific area are additionally mentioned.  Komagataella yeasts normally develop as haploid cells and are secondary homothallic as they will change mating sort.

Green recycling process for polyurethane foams by a chem-biotech approach

Green recycling process for polyurethane foams by a chem-biotech approach

Polyurethanes are extremely resistant supplies used for constructing insulation or automotive seats. The polyurethane end-of-life situation should be addressed by the event of environment friendly recycling strategies. Since typical recycling processes usually are not appropriate for thermosets, waste administration of PU foam is especially questioning. By coupling organic and chemical processes, this research goals at creating a inexperienced recycling pathway for PU foam utilizing enzymes for depolymerization. For occasion, enzymatic degradation of a PU foam synthesized with polycaprolactone and toluene diisocyanate led to a weight lack of 25 % after 24 h of incubation.

The corresponding degradation merchandise have been recovered and recognized as 6-hydroxycaproic acid and a quick acid-terminated diurethane. An organo-metallic catalyzed synthesis of second technology polymers from these constructing blocks was carried out. A polymer with a excessive common molar mass of 74 000 (Mw) was obtained by mixing 50 % of recycled constructing blocks and 50 % of neat 6-hydroxycaproic acid. A poly(ester urethane) have been synthesized with out using poisonous and decrier polyisocyanates. It is the primary time that a research presents the imaginative and prescient of a recycling loop ranging from PU wastes and ending with a second technology polymer in a full round approach

mRNA applied sciences have the potential to remodel areas of medication, together with the prophylaxis of infectious ailments. The benefits for vaccines vary from the acceleration of immunogen discovery to speedy response and a number of illness goal manufacturing. A higher understanding of high quality attributes that dictate translation effectivity, in addition to a complete appreciation of the significance of mRNA supply, are influencing a new period of funding in growth actions. The utility of translational sciences and rising early-phase scientific expertise proceed to tell candidate vaccine choice. Here we evaluation the state-of-the-art for the prevention of infectious ailments by utilizing mRNA and pertinent matters to the biotechnology and pharmaceutical industries.

Quadrivalent Influenza Vaccine (Sinovac Biotech) for seasonal influenza prophylaxis

Quadrivalent Influenza Vaccine (Sinovac Biotech) is a quadrivalent split-virion inactivated influenza vaccine authorized in China in June 2020 for people ≥three years of age. It comprises 15 µg hemagglutinin per pressure together with A/H1N1, A/H3N2, B/Victoria, and B/Yamagata, which might probably enhance safety in opposition to influenza B viruses. In this evaluation, we summarize the event of quadrivalent influenza vaccines in China and overseas nations, and assess the immunogenicity and security from the section I and III scientific trials of Quadrivalent Influenza Vaccine in people ≥three years of age. We additionally talk about the potential utility of Quadrivalent Influenza Vaccine in younger kids 6-35 months of age based on the outcomes of the section III trial.
The immunogenicity and security profiles of Quadrivalent Influenza Vaccine containing two A and two B strains have been corresponding to the trivalent vaccines for the shared strains. The addition of a second B pressure to the trivalent vaccine might induce superior immune responses for the alternate B pressure. Since the 2 B strains co-circulated worldwide, the introduction of quadrivalent influenza vaccines has been anticipated to be a cost-effective technique. We in contrast the monetary efficiency of 319 BIOTECH corporations targeted on creating therapeutics with IPOs from 1997-2016, to that of paired, non-biotech CONTROL corporations with concurrent IPO dates. Both cohorts exhibited a high-risk/excessive reward sample of return, with the bulk shedding worth, however many reaching progress multiples. 
BIOTECH corporations had a distinctly totally different monetary construction with excessive R&D expense, little income, and unfavorable income (losses), however a comparable length of itemizing on public markets and frequency of acquisitions. Through 2016, BIOTECH and CONTROL corporations had equal progress in market cap and shareholder worth (>$100 billion), however BIOTECH corporations had decrease internet worth creation ($93 billion vs $411 billion). While investments in biotechnology are sometimes thought-about to be distinctively dangerous, we conclude that worth creation by biotech corporations after IPO resembles that of non-biotech corporations at a comparable stage and doesn’t current a disproportionate funding threat.
Green recycling process for polyurethane foams by a chem-biotech approach

Swiss Startup Framework: A Highly Effective Network Supporting the Generation of Emerging Biotech Businesses

For over 50 years, Switzerland has been one of many main nations driving innovation in biotechnology and its industrial purposes. Today, some 1,000 biotech corporations kind a tightly knit, cross-functional community starting from analysis by means of to manufacturing. This community contains R&D corporations, contract analysis organizations, and extremely specialised advisors and biotech traders. Together, they kind an exterior innovation pool that enhances the in-house R&D capability of the massive multi-national pharma corporations. A extremelyefficient startup framework, strong acceleration mechanisms, and progressive traders allow the emergence of a steady stream of biotech startups that revitalize the trade with new applied sciences and merchandise supporting drug growth and diagnostics.

Phosphate Assay Kit (Fluorometric)

K2076-100 100 assays
EUR 363

QuantiChrom Phosphate Assay Kit

DIPI-500 500
EUR 372
Description: Quantitative determination of phosphate by colorimetric (620nm) method. Procedure: 30 min. Kit size: 500 tests. Detection limit: 3 µg/dL (0.3 µM). Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

PiBlue Phosphate Assay Kit

POPB-500 500
EUR 254
Description: High-throµghput phosphate assay using malachite green method at 620nm. Kit size: 500 tests. Detection limit: 0.4 µM. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

Phosphate Colorimetric Assay Kit

K410-500
EUR 316

Phosphate Assay Kit (Fluorometric)

K420-100
EUR 349

PicoProbe Phosphate Assay Kit

55R-1403 100 assays
EUR 621
Description: Assay Kit for detection of Phosphate in the research laboratory

Phosphate Fluorometric Assay Kit

55R-1404 100 assays
EUR 438
Description: Assay Kit for detection of Phosphate in the research laboratory

PBlue Phosphate Assay Kit

Z5030013 500 assays
EUR 389
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Phosphoprotein Phosphate Estimation Assay Kit

BSP061 2000Assays, 2000preps
EUR 91.76

Sucrose Phosphate Synthase Assay Kit

abx298805-100Assays 100 Assays
EUR 629

Malachite Green Phosphate Assay Kit

abx298878-100Assays 100 Assays
EUR 378

Malachite Green Phosphate Assay Kit

POMG-25H 2500
EUR 275
Description: High-throµghput phosphate assay using malachite green method at 620nm. Kit size: 2500 tests. Detection limit: 0.02 µM. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

PicoProbe? Phosphate Fluorometric Assay Kit

K419-100
EUR 446

Glucose-6-Phosphate Assay Kit

55R-1487 100 assays
EUR 706
Description: Assay Kit for detection of Glucose-6-Phosphate in the research laboratory

Malachite Green Phosphate Assay Kit

Z5030012 2,500 assays
EUR 342
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

MESG *Phosphate assay reagent*

21600 5 mg
EUR 115

Glucose-6-Phosphate Dehydrogenase Assay Kit

abx298812-100Assays 100 Assays
EUR 425

Glucose-6-Phosphate Colorimetric Assay Kit

K2208-100 100 assays
EUR 529

Glucose-6-Phosphate Fluorometric Assay Kit

K2220-100 100 assays
EUR 599

Fructose-6-Phosphate Fluorometric Assay Kit

K2222-100 100 assays
EUR 599

EnzyChrom Glucose-6-Phosphate Assay Kit

EG6P-100 100
EUR 405
Description: For quantitative colorimetric determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism. Method: OD460nm. Samples: plasma, serum,tissue and culture media etc. Species: all. Procedure: 20 min. Size: 100 tests. Dete

Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric)

K2018-100
EUR 588

Glucose-6-Phosphate Colorimetric Assay Kit

K657-100
EUR 512

Glucose-1-Phosphate Colorimetric Assay Kit

K697-100
EUR 550

PicoProbe Glucose-6-Phosphate Assay Kit

55R-1495 100 assays
EUR 758
Description: Assay Kit for detection of Glucose-6-Phosphate in the research laboratory

PicoProbe Fructose-6-Phosphate Assay Kit

55R-1496 100 assays
EUR 758
Description: Assay Kit for detection of Fructose-6-Phosphate in the research laboratory

Glucose-6-Phosphate Dehydrogenase Assay Kit

55R-1520 100 assays
EUR 706
Description: Assay Kit for detection of Glucose-6-Phosphate Dehydrogenase in the research laboratory

Glycerol-3-Phosphate (G3P) Assay Kit (Colorimetric)

MET-5075 100 assays
EUR 473

Glycerol-3-Phosphate (G3P) Assay Kit (Fluorometric)

MET-5076 100 assays
EUR 508

PicoProbeTM Glycerol-3-Phosphate Assay Kit (Fluorometric)

K196-100