Novel coronavirus (2019-nCoV)

The recent emergence of a novel coronavirus (2019-nCoV), which caused an outbreak of unusual viral pneumonia in tens of people in Wuhan, a central city of China, restated the risk of coronaviruses posed to public health. In this mini-review, we give a brief introduction of the general features of coronaviruses and describe various diseases caused by different coronaviruses in humans and animals. This review will help understand the biology and potential risk of coronaviruses that exist in richness in wildlife such as bats.

2019-nCoV qPCR Kit

Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit



Nucleic Acid:RNA
Packing (tests/kit):25

Product Details

Cat.# Instrument* Quantity Price
RR-0478-02 Ⅲ,Ⅳ – 25 primer sets 1250 Euro Gentaur

Protocol for nCorV rt-pcr detection

  • Ⅰ:LightCycler 1.0 (Internal control can’t be used for this system)
  • Ⅱ:LightCycler 2.0
  • Ⅲ:PE5700, MJ-Opticon & other single color systems
  • Ⅳ:ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems

NatTrol Control solution

nCorV Rna extraction

Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.)

Identification of nCorV Rna

On January 11, 2020, Chinese health authorities preliminary identified more than 40 human infections with a novel corona-virus in an outbreak of pneumonia under investigation in Wuhan City, Hubei Province, China. The Chinese authorities identified a new type of corona virus (novel coronavirus,named as 2019-nCoV), which was isolated on 7 January 2020. Corona-viruses are a large family of viruses, some causing illness in human and others circulating among animals such as camels, cats and bats. 2019-nCoV is a novel corona virus. The primer and probe design for this kit is based on the newly released strain (2019-nCoV) (GeneBank accession: MN908947) and covers 6 2019-nCoV strains sequences (EPI_ISL_402119, EPI_ISL_402120, EPI_ISL_402121,EPI_ISL_402122,EPI_ISL_402123, EPI_ISL_402124included). The kit contains a specific ready-to-use system for the detection of Novel Corona virus (2019-nCoV) by Reverse transcription Polymerase Chain Reaction (RT-PCR) in the real-time PCR system. The master contains a Super Mix forthe specific amplification of virus RNA. The reaction is done inone step real time RT-PCR. The first step is a reverse transcription (RT), during which the virus RNAis transcribed into cDNA. Afterwards, a thermostable DNApolymerase is used to amplify the specific gene fragments by means of polymerase chain reaction (PCR). Fluorescence is emitted and measured by the real time systems´ optical unit during PCR. The detection of amplified virus DNA fragment is performed in fluorimeter channel FAM, HEX/VIC/JOE and Cal Red 610/ ROX/TEXASRED with the fluorescentquencher BHQ1.

The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially (Ct) is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities in real time allows the detection

(2019-nCoV) Real Time Multiplex RT-PCR Kit

Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit (Detection for 3 Genes )


Nucleic Acid:RNA
Packing (tests/kit):25 Inquiry
(Under Development)

Product Details


Cat.# Instrument* Quantity Price
RR-0479-02 Ⅲ,Ⅳ 1 primer set 1250 euro from Gentaur

Thermo-cyclers list

  • Ⅰ:LightCycler 1.0 (Internal control can’t be used for this system)
  • Ⅱ:LightCycler 2.0
  • Ⅲ:PE5700, MJ-Opticon & other single color systems
  • Ⅳ:ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems 
  • V Cepheid GenExpert

Positive Control:

Coronavirus NatTrol

Other NatTrols

Carry Lam explaining the importance of early nCorV detection to increase prevention efficiency
Carry Lam explaining the importance of early nCorV detection to increase prevention efficiency

What infections can you expect in European Airports in 2020?

Are there risks of epidemics breaking out in the holliday season in Europe in 2020?

Incidentally, it remains difficult to prove causality, because Western Europeans will travel so often to tropical countries in 2020.

Infections present in Europe through tourism are West Nile Fever, dengue fever (dengue), infections by ricketsiae, and not to be missed: Entamoeba histolitica.

What deseases are unlikely to contaminate European Airports?

You shouldn’t immediately fear Ebola either. HIV, that depends on which cup you put your cookie on … AND then you are more likely to have hepatitis B or C.

Can traveling within Europe be a health risk too?

The ecology of Europe is changing. In 2020 we are getting more and more risk of a local fox tapeworm infection (never eat low blackberries in an area where foxes live).

Can I eat fresh fish in Europe in 2020?

There is also the herring worm that you can incur when eating Dutch or Danish “maatjes” or raw herring.

There is Listeria in 2020 that can also occur in smoked fish (!!!) Not only artisannal French or Italian cheese or dried meat products are known to possibly contain since long Listeria.

Is there a risk of Cholera or Typhus in Europe in 2020?

Cholera, typhoid fever and plague will stay out of Europe for the time being. There was a Cholera outbreack in Algeria in 2019 through vegetables irrigated with contaminated sewage water of toilets.

Zika virus could always be related to traveling in South America. Europe is endemic Zika free in 2020.

Pork is relatively safe in Europe in 2020 … even for roundworms … (Relative !!! ).

What will be the risk of Lime disease or Borelliosis travelling in 2020 in Europe?

Borreliosis (Lyme disease) is also on the rise again in Souther Belgium, France, Germany. Never walk barefeet or with sandals in grass during the 2020 summer if foxes or wild boar are known in the area. We advide high walking shoes with high socks or high walking boots or wellies. Neoprene wellies are easier to walk on long distances in nature. Not all teaks are contaminated with Lime disease. If you see after a bite a double circle around the biting area within 2 days you should consult a doctor for Pcr diagnosis of Borellia.

Is there a risk of Tuberculosis in Europe?

Tuberculosis fast testing with Ultra sensitive ELisa

Worse is tuberculosis in Eastern, Western Europe, India and Noth Africa. Both Mycobacterium tuberculosis and M. bovis appear to be in resurgence in 2020. Tuberculosis strains are often antibiotic resistant and a Gentaur spoligotyping test can very fast determine what antibiotic to take.

How often do I have to wash my hands traveling in Europe?

Imortant to know: every bacterium has a ‘porte d’entrée’, a minimal infectious population of intake (which is in combined to the individual immunity of ‘the victim). Washing more often your hands when traveling through European Airport Hubs is advisable in 2020.

With staphylococci and streptococci & co, the minimal number of bacteria that can only infect you are quite high: 10 e3 to 10 e5. Less than 1000 to 100 000 bacteria wil not infect you. Washing hands often is advised because Staphylococcus Aureus or the hospital bacteria is getting more and more antibiotic resistant. Multi Drug Resistant S. Aureus is especially dangerouse when you are travelling with an open wound. Cover the wound and wash your hands often.

For Mycobacteria you need only 1 (one) bacterium to get infected. Especially dangerouse after your trip because they take a long time to come to symptoms … But then you are really in trouble.

This aricle is written by:

Lieven Gevaert, bio-engineer at Gentaur Institute and frequent traveller.